产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Histone H3 (mono methyl R17) HIST1H3A Rabbit Monoclonal Antibody
目录 :
M12477-4
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
BDI-8
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
图像
图像 1 :
武汉博士德生物工程有限公司 M12477-4 图像 1
Western blot analysis of Histone H3 (mono methyl R17) expression in HeLa cell lysate (M12477-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H3A monoclonal antibody (Catalog # M12477-4) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H3A
图像 2 :
武汉博士德生物工程有限公司 M12477-4 图像 2
Immunohistochemical analysis of paraffin-embedded mouse colon, using Histone H3 (mono methyl R17) Antibody(M12477-4). HIST1H3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H3A Antibody (M12477-4)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
M12477-4
产品名称 :
Anti-Histone H3 (mono methyl R17) HIST1H3A Rabbit Monoclonal Antibody
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
BDI-8
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
IF, 免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:100-1:2000。 免疫组化1:100-1:250。 免疫细胞化学/IF 1:100-1:250
描述 :
Boster Bio Anti-Histone H3 (mono methyl R17) HIST1H3A Rabbit Monoclonal Antibody catalog # M12477-4. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
HIST1H3A
UniProt数据库编号 :
P68431
免疫原 :
A synthesized peptide derived from human Histone H3 (mono methyl R17)
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
组蛋白H3.1
同义词 :
组蛋白H3.1;组蛋白H3/a;组蛋白H3/b;组蛋白H3/c;组蛋白H3/d;组蛋白H3/f;组蛋白H3/h;组蛋白H3/i;组蛋白H3/j;组蛋白H3/k;组蛋白H3/l;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;
分子量 :
15404分子量
Protein Function :
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Localization :
Nucleus. Chromosome.
研究种类 :
ChIP’ing Antibodies, Epigenetics and Nuclear Signaling, Histones, Unmodified
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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