产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Histone H4 (tri methyl K20) HIST1H4A Rabbit Monoclonal Antibody
目录 :
M14495
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
DHB-8
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
图像
图像 1 :
Western blot analysis of Histone H4 (tri methyl K20) expression in HeLa cell lysate (M14495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIST1H4A monoclonal antibody (Catalog # M14495) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIST1H4A
图像 2 :
Immunohistochemical analysis of paraffin-embedded human stomach, using Histone H4 (tri methyl K20) Antibody(M14495). HIST1H4A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HIST1H4A Antibody (M14495)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
M14495
产品名称 :
Anti-Histone H4 (tri methyl K20) HIST1H4A Rabbit Monoclonal Antibody
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
DHB-8
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
免疫组化, 免疫印迹
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:200
描述 :
Boster Bio Anti-Histone H4 (tri methyl K20) HIST1H4A Rabbit Monoclonal Antibody catalog # M14495. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
HIST1H4A
UniProt数据库编号 :
P62805
免疫原 :
A synthesized peptide derived from human Histone H4 (tri methyl K20)
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
组蛋白H4
同义词 :
组蛋白H4;HIST1H4A;H4/A, H4FA;HIST1H4B;H4/I, H4FI;HIST1H4C;H4/G, H4FG;HIST1H4D;H4/B, H4FB;HIST1H4E;H4/J, H4FJ;HIST1H4F;H4/C, H4FC;HIST1H4H;H4/H, H4FH;HIST1H4I;H4/M, H4FM;HIST1H4J;H4/E, H4FE;HIST1H4K;H4/D, H4FD;HIST1H4L;H4/K, H4FK;HIST2H4A;H4/N, H4F2, H4FN, HIST2H4;HIST2H4B;H4/O, H4FO;HIST4H4;
分子量 :
11367分子量
Protein Function :
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Localization :
Nucleus. Chromosome.
研究种类 :
ChIP’ing Antibodies, Epigenetics and Nuclear Signaling, Histones, Unmodified
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits