免疫印迹, 免疫组化, 免疫细胞化学
图像 1 :
Western blot analysis of Cytokeratin 6 expression in A431 cell lysate (M30943-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT6A monoclonal antibody (Catalog # M30943-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT6A
IF, 免疫组化, 免疫细胞化学, 免疫印迹
免疫印迹1:500-1:2000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200
Boster Bio Anti-Cytokeratin 6 Rabbit Monoclonal Antibody catalog # M30943-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
A synthesized peptide derived from human Cytokeratin 6
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Keratin, type II cytoskeletal 6A
Keratin, type II cytoskeletal 6A; Cytokeratin-6A; CK-6A; Cytokeratin-6D; CK-6D; Keratin-6A; K6A; Type-II keratin Kb6; Hom s 5; KRT6A; K6A, KRT6D;
Protein Function :
Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.
Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.
Class I, Cytoskeleton, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Intermediate Filaments, Microtubules, Signal Transduction, Structures
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