抗体

Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100)

MA1004

100μg/vial

315美元

单克隆

小鼠

未共轭

Bcl-2-100

人类, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100)

315美元

100μg/vial

单克隆

Bcl-2-100

小鼠

人类

鸡

免疫组化, 免疫细胞化学, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 0.4-1µg/ml, Human, By Heat. Immunocytochemistry, 1µg/ml, HumanImmunohistochemistry (Frozen Section), 0.4-1µg/ml, Human Western blot, 1-2µg/ml, Human.

Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100) catalog # MA1004. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

BCL2

P49950

Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.

冻干

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

腹水

No cross reactivity with other proteins

小鼠IgG1

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Apoptosis regulator Bcl-2

Apoptosis regulator Bcl-2; Bcl2; Bcl-2;

Apoptosis regulator Bcl-2

26622分子量

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (By similarity).

Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

Expressed in a variety of tissues, with highest levels in reproductive tissues. In the adult brain, expression is localized in mitral cells of the olfactory bulb, granule and pyramidal neurons of hippocampus, pontine nuclei, cerebellar granule neurons, and in ependymal cells. In prenatal brain, expression is higher and localized in the neuroepithelium and in the cortical plate.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.

Belongs to the Bcl-2 family.

Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t (14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.

Apoptosis, Apoptotic Markers, Cancer, Cancer Metabolism, Cell Biology, Cell Death, Hypoxia, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oncoproteins, Oncoproteins/Suppressors, Pathways And Processes, Response To Hypoxia, Signal Transduction, Tumor Biomarkers