产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗GFAP抗体(单克隆, G-A-5)
目录 :
MA1045
规格 :
100μg/vial
价格 :
315美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
G-A-5
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
图像
图像 1 :
Anti- GFAP antibody, MA1045, IHC(P). IHC(P): Rat Brain Tissue
图像 2 :
Figure 2. IF analysis of GFAP, MBP using anti- GFAP, MBP antibody (MA1045, PA1050) . GFAP, MBP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- GFAP, MBP Antibody (MA1045, PA1050) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126), Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
产品信息
SKU号 :
MA1045
产品名称 :
抗GFAP抗体(单克隆, G-A-5)
价格 :
315美元
规格 :
100μg/vial
克隆性 :
单克隆
克隆编号 :
G-A-5
宿主 :
小鼠
反应物种 :
人类, 猪, 大鼠
应用(s) :
IF, 免疫组化-P, 免疫组化-F, 免疫印迹
应用细节 :
Western blot, 0.5-1µg/ml, Human, pig, rat.
Immunohistochemistry (Paraffin-embedded Section), 0.4-1µg/ml, Human, pig, rat, By Heat.
Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Human, pig, rat.
Immunofluorescence, 2µg/ml, Rat.
应用注释 :
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-GFAP Antibody (Monoclonal, G-A-5) catalog # MA1045. Tested in IF, IHC-P, IHC-F, WB applications. This antibody reacts with Human, Pig, Rat.
浓度 :
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
基因名称 :
GFAP
UniProt数据库编号 :
P47819
免疫原 :
GFAP来自猪脊髓
形式 :
冻干
Contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
纯化 :
腹水
交叉活性 :
No cross reactivity with other proteins
抗体亚型 :
小鼠IgG1
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
基因全称 :
胶质纤维酸性蛋白
同义词 :
Glial fibrillary acidic protein; GFAP; Gfap;
蛋白名 :
胶质纤维酸性蛋白
分子量 :
49957分子量
Protein Function :
GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subcellular Localization :
Cytoplasm. Associated with intermediate filaments.
组织特异性 :
Expressed in the cortex and hippocampus. Expression decreases following acute and chronic corticosterone treatment.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
Sequence Similarities :
Belongs to the intermediate filament family.
背景 :
Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.
研究种类 :
Cell Adhesion Proteins, Cell Type Marker, Cell Type Markers, Cytoskeleton, Cytoskeleton / Ecm, Intermediate Filaments, Neural Stem Cells, Neuroscience, Signal Transduction, Stem Cells, Tags & Cell Markers
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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