产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗增殖细胞核抗原抗体(单克隆, PC 10)
目录 :
MA1083
规格 :
100μg/vial
价格 :
315美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
PC 10
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Figure 1. Western blot analysis of PCNA using anti- PCNA antibody (MA1083). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates,. Lane 2: human MDA-MB-231 whole cell lysates, . Lane 3: human Jurkat whole cell lysates, . Lane 4: human HT1080 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- PCNA antigen affinity purified monoclonal antibody (Catalog # MA1083) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PCNA at approximately 35KD. The expected band size for PCNA is at 29KD.
图像 2 :
Figure 2. IHC analysis of PCNA using anti- PCNA antibody (MA1083). PCNA was detected in paraffin-embedded section of human Rectal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti- PCNA Antibody (MA1083) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
图像 3 :
Figure 3. IHC analysis of PCNA using anti- PCNA antibody (MA1083). PCNA was detected in immunocytochemical section of human HELA Cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti- PCNA Antibody (MA1083) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
产品信息
SKU号 :
MA1083
产品名称 :
抗增殖细胞核抗原抗体(单克隆, PC 10)
价格 :
315美元
规格 :
100μg/vial
克隆性 :
单克隆
克隆编号 :
PC 10
宿主 :
小鼠
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
牛
应用(s) :
免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.4-1µg/ml, Human, mouse, rat, By Heat.
Immunocytochemistry, 1µg/ml, Human, mouse, ratImmunohistochemistry (Frozen Section), 0.4-1µg/ml, Human, mouse, rat Western blot, 2µg/ml, Human, mouse, rat.
应用注释 :
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-PCNA Antibody (Monoclonal, PC 10) catalog # MA1083. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
基因名称 :
增殖细胞核抗原
UniProt数据库编号 :
P04961
免疫原 :
蛋白质A融合蛋白
形式 :
冻干
Contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
纯化 :
腹水
交叉活性 :
No cross reactivity with other proteins
抗体亚型 :
小鼠IgG2a
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
基因全称 :
增殖细胞核抗原
同义词 :
增殖细胞核抗原;增殖细胞核抗原;细胞周期蛋白;增殖细胞核抗原;
蛋白名 :
增殖细胞核抗原
分子量 :
28749分子量
Protein Function :
Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity).
Subcellular Localization :
Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (By similarity).
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
Sequence Similarities :
Belongs to the PCNA family.
背景 :
Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA/cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.
研究种类 :
Cancer, Cell Biology, Cell Cycle, Cell Division, Cell Type Markers, Chromatin Binding Proteins, DNA / RNA, DNA / RNA Binding, DNA Synthesis, Epigenetics and Nuclear Signaling, Tags & Cell Markers, Tumor Antigens, Tumor Biomarkers
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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