产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗PKB甲抗体(单克隆, PKB-175)
目录 :
MA1085
规格 :
100 ug/vial
价格 :
240美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
PKB-175
反应物种 :
人类, 小鼠, 大鼠,
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 MA1085 图像 1
Anti- PKB alpha antibody, MA1085, Western blotting. All lanes: Anti PKB alpha (MA1085) at 0.5ug/ml. Lane 1: Rat Ovary Tissue Lysate at 50ug. Lane 2: PC-12 Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: MCF-7 Whole Cell Lysate at 40ug. Lane 5: HEPA Whole Cell Lysate at 40ug. Predicted bind size: 56KD. Observed bind size: 56KD
产品信息
SKU号 :
MA1085
状态 :
Enabled
名称 :
抗PKB甲抗体(单克隆, PKB-175)
目录名称 :
一抗, 单克隆抗体
基因名称 :
AKT1
价格 :
240美元
price various sizes :
30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1050 / $240 100ug+Free Biotin Secondary BA1001 / $240
克隆性 :
单克隆
克隆编号 :
PKB-175
浓度 :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
共轭标签 :
No
contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
描述 :
Monoclonal clone# G2 antibody for AKT1 detection. Host: Mouse.Size: 100ug/vial. Tested applications: WB. Reactive species: Chicken. AKT1 information: Molecular Weight: 55735 MW; Subcellular Localization: Cytoplasm . Nucleus . Cell membrane . Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A (By similarity). Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (By similarity); Tissue Specificity: Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis.
short description :
Mouse IgG monoclonal antibody for PKB alpha, v-akt murine thymoma viral oncogene homolog 1 (AKT1) detection. Tested with WB in Human;mouse;rat;chicken. No cross reactivity with other proteins.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P47196
宿主 :
小鼠
免疫原 :
合成多肽对应于氨基酸461-477人的PKB甲/Akt1, , KLH偶联
形式 :
冻干
纯化 :
腹水
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
抗体亚型 :
小鼠IgG1
重组 :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
应用细节 :
免疫印迹, 0.25 0.5微克/毫升, 人类, 小鼠, 大鼠, 鸡
应用 :
免疫印迹
反应物种 :
鸡, 人类, 小鼠, 大鼠
前体活性 :
仓鼠
图像标记 :
Anti- PKB alpha antibody, MA1085, Western blotting. All lanes: Anti PKB alpha (MA1085) at 0.5ug/ml. Lane 1: Rat Ovary Tissue Lysate at 50ug. Lane 2: PC-12 Whole Cell Lysate at 40ug. Lane 3: A549 Whole Cell Lysate at 40ug. Lane 4: MCF-7 Whole Cell Lysate at 40ug. Lane 5: HEPA Whole Cell Lysate at 40ug. Predicted bind size: 56KD. Observed bind size: 56KD
背景 :
PKB alpha also knows as V-AKT murine thymoma vial oncogene homolog 1(ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.
competitor equivalent skus :
sc 271149 sc 1618 sc 5298 sc 55523 sc 7126 sc 377457 sc 135829 sc 1618 R
研究种类 :
Apoptosis, Cancer, Cell Cycle, Cell Death, Epigenetics And Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear, Pathways And Processes, Protein Phosphorylation, Ser / Thr Kinases, Serine/Threonine Kinases, Signal Transduction
同义词 :
RAC甲丝氨酸/苏氨酸蛋白激酶;2.7。 11.1;蛋白激酶B;PKB;蛋白激酶B甲;PKB甲;RAC-PK;Akt1;
基因全称 :
RAC甲丝氨酸/苏氨酸蛋白激酶
分子量 :
55735分子量
protein function :
AKT1 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)- response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr- 117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation (By similarity). Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity (By similarity). Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53 (By similarity).
subcellular localization :
Cytoplasm . Nucleus . Cell membrane . Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A (By similarity). Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. Colocalizes with WDFY2 in intracellular vesicles (By similarity).
组织特异性 :
Widely expressed. Low levels found in liver with slightly higher levels present in thymus and testis.
蛋白名 :
RAC甲丝氨酸/苏氨酸蛋白激酶
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
sequence similarities :
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
更新 :
1/28/19 19:39
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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