产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Tropomyosin(Sarcomeric) Tpm1 Antibody (Monoclonal, CH1)
目录 :
MA1097
规格 :
100μg/vial
价格 :
315美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
CH1
反应物种 :
小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
图像
图像 1 :
Figure 1. Western blot analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody (MA1097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, . Lane 2: rat skeletal muscle tissue lysates, . Lane 3: mouse heart tissue lysates,. Lane 4: mouse skeletal muscle tissue lysates,. Lane 5: human Hela whole cell lysates,. Lane 6: human U2OS whole cell lysates,. Lane 7: human A549 whole cell lysates,. Lane 8: human Caco-2 whole cell lysates,. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Tropomyosin(Sarcomeric) antigen affinity purified monoclonal antibody (Catalog # MA1097) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tropomyosin(Sarcomeric) at approximately 39KD. The expected band size for Tropomyosin(Sarcomeric) is at 33KD.
图像 2 :
Figure 2. IHC analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody (MA1097). Tropomyosin(Sarcomeric) was detected in paraffin-embedded section of human skeletal muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-Tropomyosin(Sarcomeric) Antibody (MA1097) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
图像 3 :
Figure 3. IHC analysis of Tropomyosin(Sarcomeric) using anti- Tropomyosin(Sarcomeric) antibody (MA1097). Tropomyosin(Sarcomeric) was detected in paraffin-embedded section of mouse skeletal muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti-Tropomyosin(Sarcomeric) Antibody (MA1097) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
产品信息
SKU号 :
MA1097
产品名称 :
Anti-Tropomyosin(Sarcomeric) Tpm1 Antibody (Monoclonal, CH1)
价格 :
315美元
规格 :
100μg/vial
克隆性 :
单克隆
克隆编号 :
CH1
宿主 :
小鼠
反应物种 :
鸡, 人类, 小鼠, 大鼠
前体活性 :
牛
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 2-4µg/ml, Human, rat, chicken, By Heat.
Immunohistochemistry (Frozen Section), 2-4µg/ml, Human, rat, chicken Western blot, 1-2µg/ml, Human, rat, chicken.
应用注释 :
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Tropomyosin (Sarcomeric) Tpm1 Antibody (Monoclonal, CH1) catalog # MA1097. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
浓度 :
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
基因名称 :
TPM1
UniProt数据库编号 :
P04692
免疫原 :
鸡肌肉原肌球蛋白
形式 :
冻干
Contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
纯化 :
腹水
交叉活性 :
No cross reactivity with other proteins
抗体亚型 :
小鼠IgG1
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
基因全称 :
原肌球蛋白甲1链
同义词 :
Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1; Tpm1; Alpha-tm, Tpma;
蛋白名 :
原肌球蛋白甲1链
分子量 :
32681分子量
Protein Function :
Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Subcellular Localization :
细胞质, 细胞骨架
组织特异性 :
TPM1: Detected in primary breast cancer tissues butundetectable in normal breast tissues in Sudanese patients.Isoform 1 is expressed in adult and fetal skeletal muscle andcardiac tissues, with higher expression levels in the cardiactissues. Isoform 10 is expressed in adult and fetal cardiactissues, but not in skeletal muscle.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
背景 :
Tropomyosin is an alpha-helical, parallel, two-chain coiled coil which binds along the length of actin filaments in both muscle and non-muscle cells. This gene associates N-terminus to C-terminus to form a continuous strand along both sides of the actin filament and regulates its function. Tropomyosin contributes to most, if not all, functions of the actin cytoskeleton, and its role is essential for the viability of a wide range of organisms. The ability of tropomyosin to contribute to the many functions of the actin cytoskeleton is related to the temporal and spatial regulation of expression of tropomyosin isoforms.
研究种类 :
Actin Etc, Cardiovascular, Cytoskeleton, Cytoskeleton / Ecm, Hypertrophy, Microfilaments, Signal Transduction
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits