产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗酪氨酸羟化酶抗体(单克隆, TH-16)
目录 :
MA1100
规格 :
100μg/vial
价格 :
315美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
TH-16
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
图像
图像 1 :
武汉博士德生物工程有限公司 MA1100 图像 1
Figure 1. IHC analysis of Tyrosine Hydroxylase using anti- Tyrosine Hydroxylase antibody (MA1100). Tyrosine Hydroxylase was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti- Tyrosine Hydroxylase Antibody (MA1100) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
图像 2 :
武汉博士德生物工程有限公司 MA1100 图像 2
Figure 2. Western blot analysis of Tyrosine Hydroxylase using anti-Tyrosine Hydroxylase antibody (MA1100). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, . Lane 2: mouse brain tissue lysate, . Lane 3: human U-87MG cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tyrosine Hydroxylase antigen affinity purified monoclonal antibody (Catalog # MA1100) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tyrosine Hydroxylase at approximately 59KD. The expected band size for Tyrosine Hydroxylase is at 59KD.
产品信息
SKU号 :
MA1100
产品名称 :
抗酪氨酸羟化酶抗体(单克隆, TH-16)
价格 :
315美元
规格 :
100μg/vial
克隆性 :
单克隆
克隆编号 :
TH-16
宿主 :
小鼠
反应物种 :
人类, 小鼠, 兔, 大鼠
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, rat, rabbit, By Heat. Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Human, rat, rabbit Western blot, 0.25-0.5µg/ml, Human, mouse, rat, rabbit.
应用注释 :
Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Tyrosine Hydroxylase Antibody (Monoclonal, TH-16) catalog # MA1100. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rabbit, Rat.
浓度 :
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
基因名称 :
TH
UniProt数据库编号 :
P04177
免疫原 :
大鼠酪氨酸羟化酶(TH)
形式 :
冻干
Contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
纯化 :
腹水
交叉活性 :
No cross reactivity with other proteins
抗体亚型 :
小鼠IgG1
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
基因全称 :
酪氨酸3单加氧酶
同义词 :
Tyrosine 3-monooxygenase; 1.14.16.2; Tyrosine 3-hydroxylase; TH; Th;
蛋白名 :
酪氨酸3单加氧酶
分子量 :
55966分子量
Protein Function :
Plays an important role in the physiology of adrenergic neurons.
组织特异性 :
TH: Mainly expressed in the brain and adrenalglands.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
Sequence Similarities :
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
背景 :
Tyrosine hydroxylase is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Human TH gene contains 13 primary exons and spans approximately 8 kb. TH is in the 11p15.5 region.
研究种类 :
Cancer, Cancer Metabolism, Cell Type Marker, Endocrine Metabolism, Hormone Biosynthesis, Hypoxia, Metabolic Signaling Pathway, Metabolism, Metabolism Processes, Neuron Marker, Neuroscience, Neurotransmitter, Pathways And Processes, Response To Hypoxia
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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