产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗弹性蛋白抗体(单克隆, V9)
目录 :
MA1102
规格 :
100μg/vial
价格 :
315美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
V9
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片
更多信息或购买 :
图像
图像 1 :
Figure 1. IHC analysis of Vimentin using anti- Vimentin antibody (MA1102). Vimentin was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Vimentin Antibody (MA1102) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
Figure 2. Western blot analysis of Vimentin using anti-Vimentin antibody (MA1102). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, . Lane 2: mouse lung tissue lysates, . Lane 3: mouse spleen tissue lysates, . Lane 4: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vimentin antigen affinity purified polyclonal antibody (Catalog # MA1102) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vimentin at approximately 54KD. The expected band size for Vimentin is at 54KD.
产品信息
SKU号 :
MA1102
产品名称 :
抗弹性蛋白抗体(单克隆, V9)
价格 :
315美元
规格 :
100μg/vial
克隆性 :
单克隆
克隆编号 :
V9
宿主 :
小鼠
反应物种 :
人类, 猪, 兔, 大鼠
前体活性 :
Monkey
应用(s) :
IF, 免疫组化-P, 免疫印迹
应用细节 :
Western blot, 0.5-1µg/ml, Human, rabbit, pig, rat.
Immunohistochemistry (Paraffin-embedded Section), 1.5-2.5µg/ml, Human, rabbit, pig, rat, By Heat.
Immunofluorescence, 5µg/ml, Human
应用注释 :
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Vimentin Antibody (Monoclonal, V9) catalog # MA1102. Tested in IF, IHC-P, WB applications. This antibody reacts with Human, Pig, Rabbit, Rat.
浓度 :
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
基因名称 :
弹性蛋白
UniProt数据库编号 :
P31000
免疫原 :
Pig eye lens vimentin.
形式 :
冻干
Contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
纯化 :
腹水
交叉活性 :
No cross reactivity with other proteins
抗体亚型 :
小鼠IgG1
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
基因全称 :
弹性蛋白
同义词 :
弹性蛋白;弹性蛋白;
蛋白名 :
弹性蛋白
分子量 :
53733分子量
Protein Function :
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Subcellular Localization :
Nucleus matrix. Cytoplasm.
组织特异性 :
VIM: Highly expressed in fibroblasts, someexpression in T- and B-lymphocytes, and little or no expression inBurkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
Sequence Similarities :
Belongs to the intermediate filament family.
背景 :
The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.
研究种类 :
Neuroscience, Signal Transduction, Stem Cells, Tumor Biomarkers
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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