公司名称 :
产品类型 :
产品名称 :
抗甲平滑肌肌动蛋白大动脉平滑肌肌动蛋白alpha2抗体(单克隆, 1A4)
目录 :
规格 :
价格 :
克隆性 :
宿主 :
共轭标签 :
克隆名称 :
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
文章摘录数: 23
Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, et al. CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages. Prostaglandins Other Lipid Mediat. 2015;116-117:64-75 pubmed 出版商
Li Q, Zhu Y, Zhang M, Chen L, Zhang Z, Du Y, et al. Chlorogenic acid inhibits hypoxia-induced pulmonary artery smooth muscle cells proliferation via c-Src and Shc/Grb2/ERK2 signaling pathway. Eur J Pharmacol. 2015;751:81-8 pubmed 出版商
Qiu X, Fu K, Zhao X, Zhang Y, Yuan Y, Zhang S, et al. Protective effects of astaxanthin against ischemia/reperfusion induced renal injury in mice. J Transl Med. 2015;13:28 pubmed 出版商
Liu Z, Xu S, Huang X, Wang J, Gao S, Li H, et al. Cryptotanshinone, an orally bioactive herbal compound from Danshen, attenuates atherosclerosis in apolipoprotein E-deficient mice: role of lectin-like oxidized LDL receptor-1 (LOX-1). Br J Pharmacol. 2015;172:5661-75 pubmed 出版商
Xu G, Wang X, Deng C, Teng X, Suuronen E, Shen Z, et al. Injectable biodegradable hybrid hydrogels based on thiolated collagen and oligo(acryloyl carbonate)-poly(ethylene glycol)-oligo(acryloyl carbonate) copolymer for functional cardiac regeneration. Acta Biomater. 2015;15:55-64 pubmed 出版商
Zhao L, Yang G, Zhao X. Rho-associated protein kinases play an important role in the differentiation of rat adipose-derived stromal cells into cardiomyocytes in vitro. PLoS ONE. 2014;9:e115191 pubmed 出版商
Yang L, Liu J, Bai X, Fan L, Han F, Jia W, et al. Acute downregulation of miR-155 at wound sites leads to a reduced fibrosis through attenuating inflammatory response. Biochem Biophys Res Commun. 2014;453:153-9 pubmed 出版商
Tang J, Huang H, Ji X, Zhu X, Li Y, She M, et al. Involvement of IL-13 and tissue transglutaminase in liver granuloma and fibrosis after schistosoma japonicum infection. Mediators Inflamm. 2014;2014:753483 pubmed 出版商
Tao H, Yang J, Chen Z, Xu S, Zhou X, Zhan H, et al. DNMT3A silencing RASSF1A promotes cardiac fibrosis through upregulation of ERK1/2. Toxicology. 2014;323:42-50 pubmed 出版商
You H, Wei L, Sun W, Wang L, Yang Z, Liu Y, et al. The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats. Int J Mol Med. 2014;34:92-102 pubmed 出版商
Liu T, Zeng Z, Liu Y, Wang J, Maitz M, Wang Y, et al. Surface modification with dopamine and heparin/poly-L-lysine nanoparticles provides a favorable release behavior for the healing of vascular stent lesions. ACS Appl Mater Interfaces. 2014;6:8729-43 pubmed 出版商
Jia Z, Tian H, Kang K, Miao H, Liu K, Jiang S, et al. Expression of the tissue inhibitor of metalloproteinase-3 by transplanted VSMCs modifies heart structure and function after myocardial infarction. Transpl Immunol. 2014;30:149-58 pubmed 出版商
Liu X, Qian L, Nan H, Cui M, Hao X, Du Y. Function of the transforming growth factor-?1/c-Jun N-terminal kinase signaling pathway in the action of thalidomide on a rat model of pulmonary fibrosis. Exp Ther Med. 2014;7:669-674 pubmed
Gu G, Chen T, Zhou H, Sun K, Li J. Role of Wnt/?-catenin signaling pathway in the mechanism of calcification of aortic valve. J Huazhong Univ Sci Technolog Med Sci. 2014;34:33-36 pubmed 出版商
Fan L, Hu K, Ji K, Sun Q, Xiong J, Yang L, et al. Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons. In Vitro Cell Dev Biol Anim. 2014;50:304-12 pubmed 出版商
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Zhang R, Zhou S, Li C, Wang X, Tang Y, Chen R, et al. C-reactive protein/oxidised low-density lipoprotein/?2-glycoprotein I complex promotes atherosclerosis in diabetic BALB/c mice via p38mitogen-activated protein kinase signal pathway. Lipids Health Dis. 2013;12:42 pubmed 出版商
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Zhu X, Wang Y, Yang Y, Yang R, Zhu B, Zhang Y, et al. Suppression of lipopolysaccharide-induced upregulation of toll-like receptor 4 by emodin in mouse proximal tubular epithelial cells. Mol Med Rep. 2012;6:493-500 pubmed 出版商
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图像 1 :
武汉博士德生物工程有限公司 MA1106 图像 1
Figure 1. IHC analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106). Alpha-Smooth Muscle Actin was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti- Alpha-Smooth Muscle Actin Antibody (MA1106) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1072) with DAB as the chromogen.
图像 2 :
武汉博士德生物工程有限公司 MA1106 图像 2
Figure 2. Western blot analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, . Lane 2: human placenta tissue lysates, . Lane 3: rat stomach tissue lysates,. Lane 4: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Alpha-Smooth Muscle Actin antigen affinity purified monoclonal antibody (Catalog # MA1106) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha-Smooth Muscle Actin at approximately 43KD. The expected band size for Alpha-Smooth Muscle Actin is at 43KD.
SKU号 :
产品名称 :
抗甲平滑肌肌动蛋白大动脉平滑肌肌动蛋白alpha2抗体(单克隆, 1A4)
价格 :
规格 :
克隆性 :
克隆编号 :
宿主 :
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.1-0.5µg/ml, Human, mouse, rat, By Heat. Immunohistochemistry (Frozen Section), 0.1-0.5µg/ml, Human, mouse, rat Western blot, 2µg/ml, Human, mouse, rat.
应用注释 :
Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Alpha-Smooth Muscle Actin ACTA2 Antibody (Monoclonal, 1A4) catalog # MA1106. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
基因名称 :
UniProt数据库编号 :
免疫原 :
N-terminal synthetic decapeptide of alpha-smooth muscle actin.
形式 :
Contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
纯化 :
交叉活性 :
No cross reactivity with other proteins
抗体亚型 :
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
基因全称 :
Actin, aortic smooth muscle
同义词 :
Actin, aortic smooth muscle; Alpha-actin-2; Acta2; Actsa, Actvs;
蛋白名 :
Actin, aortic smooth muscle
分子量 :
Protein Function :
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Subcellular Localization :
细胞质, 细胞骨架
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
背景 :
Ueyama et al. (1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.
研究种类 :
Cytoskeleton / Ecm, Microfilaments, Signal Transduction
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