产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗甲平滑肌肌动蛋白抗体(单克隆, 1A4)
目录 :
MA1106
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
克隆名称 :
1A4
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
文章摘录数: 23
参考文献
Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, et al. CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages. Prostaglandins Other Lipid Mediat. 2015;116-117:64-75 pubmed 出版商
Li Q, Zhu Y, Zhang M, Chen L, Zhang Z, Du Y, et al. Chlorogenic acid inhibits hypoxia-induced pulmonary artery smooth muscle cells proliferation via c-Src and Shc/Grb2/ERK2 signaling pathway. Eur J Pharmacol. 2015;751:81-8 pubmed 出版商
Qiu X, Fu K, Zhao X, Zhang Y, Yuan Y, Zhang S, et al. Protective effects of astaxanthin against ischemia/reperfusion induced renal injury in mice. J Transl Med. 2015;13:28 pubmed 出版商
Liu Z, Xu S, Huang X, Wang J, Gao S, Li H, et al. Cryptotanshinone, an orally bioactive herbal compound from Danshen, attenuates atherosclerosis in apolipoprotein E-deficient mice: role of lectin-like oxidized LDL receptor-1 (LOX-1). Br J Pharmacol. 2015;172:5661-75 pubmed 出版商
Xu G, Wang X, Deng C, Teng X, Suuronen E, Shen Z, et al. Injectable biodegradable hybrid hydrogels based on thiolated collagen and oligo(acryloyl carbonate)-poly(ethylene glycol)-oligo(acryloyl carbonate) copolymer for functional cardiac regeneration. Acta Biomater. 2015;15:55-64 pubmed 出版商
Zhao L, Yang G, Zhao X. Rho-associated protein kinases play an important role in the differentiation of rat adipose-derived stromal cells into cardiomyocytes in vitro. PLoS ONE. 2014;9:e115191 pubmed 出版商
Yang L, Liu J, Bai X, Fan L, Han F, Jia W, et al. Acute downregulation of miR-155 at wound sites leads to a reduced fibrosis through attenuating inflammatory response. Biochem Biophys Res Commun. 2014;453:153-9 pubmed 出版商
Tang J, Huang H, Ji X, Zhu X, Li Y, She M, et al. Involvement of IL-13 and tissue transglutaminase in liver granuloma and fibrosis after schistosoma japonicum infection. Mediators Inflamm. 2014;2014:753483 pubmed 出版商
Tao H, Yang J, Chen Z, Xu S, Zhou X, Zhan H, et al. DNMT3A silencing RASSF1A promotes cardiac fibrosis through upregulation of ERK1/2. Toxicology. 2014;323:42-50 pubmed 出版商
You H, Wei L, Sun W, Wang L, Yang Z, Liu Y, et al. The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats. Int J Mol Med. 2014;34:92-102 pubmed 出版商
Liu T, Zeng Z, Liu Y, Wang J, Maitz M, Wang Y, et al. Surface modification with dopamine and heparin/poly-L-lysine nanoparticles provides a favorable release behavior for the healing of vascular stent lesions. ACS Appl Mater Interfaces. 2014;6:8729-43 pubmed 出版商
Jia Z, Tian H, Kang K, Miao H, Liu K, Jiang S, et al. Expression of the tissue inhibitor of metalloproteinase-3 by transplanted VSMCs modifies heart structure and function after myocardial infarction. Transpl Immunol. 2014;30:149-58 pubmed 出版商
Liu X, Qian L, Nan H, Cui M, Hao X, Du Y. Function of the transforming growth factor-?1/c-Jun N-terminal kinase signaling pathway in the action of thalidomide on a rat model of pulmonary fibrosis. Exp Ther Med. 2014;7:669-674 pubmed
Gu G, Chen T, Zhou H, Sun K, Li J. Role of Wnt/?-catenin signaling pathway in the mechanism of calcification of aortic valve. J Huazhong Univ Sci Technolog Med Sci. 2014;34:33-36 pubmed 出版商
Fan L, Hu K, Ji K, Sun Q, Xiong J, Yang L, et al. Directed differentiation of aged human bone marrow multipotent stem cells effectively generates dopamine neurons. In Vitro Cell Dev Biol Anim. 2014;50:304-12 pubmed 出版商
Yu K, Zhu P, Dong Q, Zhong Y, Zhu Z, Lin Y, et al. Thymic stromal lymphopoietin attenuates the development of atherosclerosis in ApoE-/- mice. J Am Heart Assoc. 2013;2:e000391 pubmed 出版商
Gong C, Hou L, Zhu Y, Lv J, Liu Y, Luo L. In vitro constitution of esophageal muscle tissue with endocyclic and exolongitudinal patterns. ACS Appl Mater Interfaces. 2013;5:6549-55 pubmed 出版商
Zhang R, Zhou S, Li C, Wang X, Tang Y, Chen R, et al. C-reactive protein/oxidised low-density lipoprotein/?2-glycoprotein I complex promotes atherosclerosis in diabetic BALB/c mice via p38mitogen-activated protein kinase signal pathway. Lipids Health Dis. 2013;12:42 pubmed 出版商
Hu J, Zhou Z, Shi S, Zhu X, Wang X, Zhang W, et al. Mesenchymal stem-like cells isolated from human esophageal carcinoma and adjacent non-cancerous tissues. Oncol Lett. 2013;5:179-184 pubmed
Zhang S, Zheng Y, Yu P, Min T, Yu F, Ye C, et al. Lentiviral vector-mediated down-regulation of IL-17A receptor in hepatic stellate cells results in decreased secretion of IL-6. World J Gastroenterol. 2012;18:3696-704 pubmed 出版商
Zhu X, Wang Y, Yang Y, Yang R, Zhu B, Zhang Y, et al. Suppression of lipopolysaccharide-induced upregulation of toll-like receptor 4 by emodin in mouse proximal tubular epithelial cells. Mol Med Rep. 2012;6:493-500 pubmed 出版商
Li W, Man X, Li C, Chen J, Zhou J, Cai S, et al. VEGF induces proliferation of human hair follicle dermal papilla cells through VEGFR-2-mediated activation of ERK. Exp Cell Res. 2012;318:1633-40 pubmed 出版商
Su Y, Liu W, Ma L, Liu X, Liu Z, Zhu B. Scutellarin inhibits translocation of protein kinase C in diabetic thoracic aorta of the rat. Clin Exp Pharmacol Physiol. 2012;39:136-40 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 MA1106 图像 1
Figure 1. IHC analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106). Alpha-Smooth Muscle Actin was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti- Alpha-Smooth Muscle Actin Antibody (MA1106) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1072) with DAB as the chromogen.
图像 2 :
武汉博士德生物工程有限公司 MA1106 图像 2
Figure 2. Western blot analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, . Lane 2: human placenta tissue lysates, . Lane 3: rat stomach tissue lysates,. Lane 4: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Alpha-Smooth Muscle Actin antigen affinity purified monoclonal antibody (Catalog # MA1106) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha-Smooth Muscle Actin at approximately 43KD. The expected band size for Alpha-Smooth Muscle Actin is at 43KD.
产品信息
SKU号 :
MA1106
状态 :
Enabled
名称 :
抗甲平滑肌肌动蛋白抗体(单克隆, 1A4)
目录名称 :
一抗, 单克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
ACTN
价格 :
280美元
price various sizes :
30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1050 / $280 100ug+Free Biotin Secondary BA1001 / $280
克隆性 :
单克隆
克隆编号 :
1A4
浓度 :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
共轭标签 :
No
contents :
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
描述 :
Monoclonal clone# G2 antibody for 1867 detection. Host: Mouse.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. 1867 information: Molecular Weight: 42009 MW; Subcellular Localization: Cytoplasm, cytoskeleton.
short description :
Mouse IgG monoclonal antibody for alpha-Smooth Muscle Actin, actin, alpha 2, smooth muscle, aorta (ACTA2 ) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P62738
宿主 :
小鼠
免疫原 :
N-terminal synthetic decapeptide of alpha-smooth muscle actin.
形式 :
冻干
纯化 :
腹水
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
抗体亚型 :
小鼠IgG2a
重组 :
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
应用细节 :
Immunohistochemistry(Paraffin-embedded Section), 0.1-0.5ug/ml, Human, mouse, rat, By Heat . Immunohistochemistry(Frozen Section), 0.1-0.5ug/ml, Human, mouse, rat, -. Western blot, 2ug/ml, Human, mouse, rat.
应用 :
免疫组化, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
图像标记 :
Figure 1. IHC analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106). Alpha-Smooth Muscle Actin was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml mouse anti- Alpha-Smooth Muscle Actin Antibody (MA1106) overnight at 4°C. Biotinylated goat anti- mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1072) with DAB as the chromogen. Figure 2. Western blot analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, . Lane 2: human placenta tissue lysates, . Lane 3: rat stomach tissue lysates,. Lane 4: mouse stomach tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- Alpha-Smooth Muscle Actin antigen affinity purified monoclonal antibody (Catalog # MA1106) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti- mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha-Smooth Muscle Actin at approximately 43KD. The expected band size for Alpha-Smooth Muscle Actin is at 43KD.
背景 :
Ueyama et al.(1990) assigned the ACTSA gene to chromosome 10 by Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids. Regional mapping by in situ hybridization localized the gene to 10q22-q24.Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome. Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins.
competitor equivalent skus :
sc 53015
研究种类 :
Cytoskeleton / Ecm, Microfilaments, Signal Transduction
同义词 :
Actin, aortic smooth muscle;Alpha-actin-2;Acta2;Actsa, Actvs;
基因全称 :
Actin, aortic smooth muscle
分子量 :
42009分子量
protein function :
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
subcellular localization :
细胞质, 细胞骨架
蛋白名 :
Actin, aortic smooth muscle
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
更新 :
1/28/19 19:39
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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