产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗磷酸化STAT3(S727)兔单克隆抗体
目录 :
P00007-1
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
CAA-19
反应物种 :
人类, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
图像
图像 1 :
Western blot analysis of Phospho-STAT3 (S727) expression in A431 cell lysate treated with EGF (P00007-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 monoclonal antibody (Catalog # P00007-1) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT3
产品信息
SKU号 :
P00007-1
产品名称 :
抗磷酸化STAT3(S727)兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
CAA-19
宿主 :
兔
反应物种 :
人类, 大鼠
应用(s) :
免疫沉淀, IF, 免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:1000-1:2000。 免疫组化1:50-1:200。 免疫细胞化学/IF 1:50-1:200。 免疫沉淀1:50
描述 :
Boster Bio Anti-Phospho-STAT3 (S727) Rabbit Monoclonal Antibody catalog # P00007-1. Tested in IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
STAT3
UniProt数据库编号 :
P40763
免疫原 :
A synthesized peptide derived from human Phospho-STAT3 (S727)
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Signal transducer and activator of transcription 3
同义词 :
Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF;
分子量 :
88068分子量
Protein Function :
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)- responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity).
Subcellular Localization :
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
组织特异性 :
心, 大脑, 胎盘, 肺, 肝, 骨骼肌, 肾脏和胰腺
研究种类 :
Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, Hypertrophy, Intracellular, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Stem Cells, Surface Molecules, Transcription, Transcription Factors, Transcription Factors/Regulators
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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