产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗磷酸化表皮生长因子受体(S695)兔单克隆抗体
目录 :
P00023-4
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
GCC-5
反应物种 :
人类
应用 :
免疫印迹, 免疫细胞化学
图像
图像 1 :
武汉博士德生物工程有限公司 P00023-4 图像 1
Western blot analysis of Phospho-EGFR (S695) expression in A431 cell lysate treated with EGF (P00023-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody (Catalog # P00023-4) overnight at 4 , then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EGFR
产品信息
SKU号 :
P00023-4
产品名称 :
抗磷酸化表皮生长因子受体(S695)兔单克隆抗体
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
GCC-5
宿主 :
反应物种 :
人类
应用(s) :
IF, 免疫细胞化学, 免疫印迹
应用细节 :
免疫印迹1:1000-1:2000。 免疫细胞化学/IF 1:50-1:200
描述 :
Boster Bio Anti-Phospho-EGFR (S695) Rabbit Monoclonal Antibody catalog # P00023-4. Tested in IF, ICC, WB applications. This antibody reacts with Human.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
表皮生长因子受体
UniProt数据库编号 :
P00533
免疫原 :
A synthesized peptide derived from human EGFR
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
表皮生长因子受体
同义词 :
Epidermal growth factor receptor; 2.7.10.1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB, ERBB1, HER1;
分子量 :
134277分子量
Protein Function :
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Subcellular Localization :
Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).
组织特异性 :
Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
研究种类 :
Cancer, Growth Factors/Hormones, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tumor Biomarkers, Tyrosine Kinases
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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