产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗磷酸化鼠双微基因2 S185抗体
目录 :
P00054
规格 :
100微克
价格 :
329美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 P00054 图像 1
Western blot analysis of MDM2 expression in untreated 293T whole cell lysate (lane 1) and 293T cell treated with IGF-1 (100 ng/ml) for 20 min prior to harvest (lane 2). MDM2 at 102KD was detected using rabbit anti-MDM2 pS185 Antigen Affinity purified polyclonal antibody (Catalog # P00054). The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
产品信息
SKU号 :
P00054
状态 :
Enabled
名称 :
抗磷酸化鼠双微基因2 S185抗体
目录名称 :
一抗, 多克隆抗体
基因名称 :
鼠双微基因2
价格 :
329美元
price various sizes :
100ug / $329 100ug+Free HRP Secondary BA1054 / $329 100ug+Free Biotin Secondary BA1003 / $329
克隆性 :
多克隆
浓度 :
1.0 mg/mL by UV absorbance at 280 nm
共轭标签 :
No
contents :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
描述 :
Anti-MDM2 phospho S185 Antibody validated for WB, IP, ELISA in Human;Mouse;Rat.
short description :
Anti-MDM2 phospho S185 Antibody validated for WB, IP, ELISA in Human;Mouse;Rat.
规格 :
100微克
sample size available :
25ug for $99, contact us for details
UniProt数据库编号 :
Q00987
宿主 :
免疫原 :
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 177-198 of mouse MDM2.
形式 :
Liquid
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
应用细节 :
Western Blot, 1:500 - 1:2, 000, human, mouse, rat. Direct ELISA, 1:3, 000 - 1:12, 000, human, mouse, rat. ImmunoPrecipitation: 1:100, human, mouse, rat.
应用 :
酶联免疫吸附测定, 免疫沉淀, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Western blot analysis of MDM2 expression in untreated 293T whole cell lysate (lane 1) and 293T cell treated with IGF-1 (100 ng/ml) for 20 min prior to harvest (lane 2). MDM2 at 102KD was detected using rabbit anti-MDM2 pS185 Antigen Affinity purified polyclonal antibody (Catalog # P00054). The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
背景 :
Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.
competitor equivalent skus :
sc 56154 sc 56430 sc 965 sc 813 sc 7918 sc 5304 sc 812 sc 13161 sc 965 X
研究种类 :
Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics And Nuclear Signaling, Oncoproteins, Oncoproteins/Suppressors, P53 Pathway, Transcription, Ubiquitin & Ubiquitin Like Modifiers
同义词 :
E3 ubiquitin-protein ligase Mdm2;6.3.2.-;Double minute 2 protein;Hdm2;Oncoprotein Mdm2;p53-binding protein Mdm2;MDM2;
基因全称 :
E3泛素蛋白连接酶 鼠双微基因2
分子量 :
55233分子量
protein function :
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation.
subcellular localization :
Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
组织特异性 :
Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
更新 :
1/28/19 19:39
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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