产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗磷酸化SMAD3 S423/S425抗体
目录 :
P00059
规格 :
100微升
价格 :
339美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 P00059 图像 1
SMAD3 was detected in paraffin-embedded sections of epidermal keratinocytes at 40X using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059) at 2.5 ug/mL. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.
图像 2 :
武汉博士德生物工程有限公司 P00059 图像 2
Western blot analysis of endogenous SMAD3 expression in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF for 1 hour. Phosphorylated SMAD3 was detected using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059). Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD.
图像 3 :
武汉博士德生物工程有限公司 P00059 图像 3
Western blot analysis of SMAD3 expression in HEK293 lysate (lane 1), HeLa Lysate (lane 2), MCF-7 Lysate (lane 3), Jurkat Lysate (lane 4), A431 Lysate (lane 5), A549 Lysate (lane 6), LNCap Lysate (lane 7), MOLT-4 Lysate (lane 8), Ramos Lysate (lane 9), Raji Lsyate (lane 10), A-172 Lysate (lane 11) and NIH/3T3 Lysate (lane 12). Phosphorylated SMAD3 at 35KD was detected using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059) at 1ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
产品信息
SKU号 :
P00059
状态 :
Enabled
名称 :
抗磷酸化SMAD3 S423/S425抗体
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
SMAD3
价格 :
339美元
price various sizes :
25ug sample size / $99 100uL / $339 100uL+Free HRP Secondary BA1054 / $339 100uL+Free Biotin Secondary BA1003 / $339
克隆性 :
多克隆
浓度 :
1.0 mg/mL by UV absorbance at 280 nm
共轭标签 :
No
描述 :
Polyclonal antibody for SMAD3 detection. Host: Rabbit.Size: 100uL. Tested applications: IHC. Reactive species: Human. SMAD3 information: Molecular Weight: 48081 MW; Subcellular Localization: Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta.
short description :
Anti-SMAD3 phospho S423/phospho S425 Antibody validated for WB in Human.
规格 :
100微升
sample size available :
25ug for $99, contact us for details
UniProt数据库编号 :
P84022
宿主 :
形式 :
Liquid
交叉活性 :
No cross reactivity with other proteins.
应用细节 :
Western Blot, 1:2, 000 - 1:20, 000, human, mouse, rat, Xenopus laevis, Xenopus tropicalis, zebrafish, swine, bovine, chicken. Flow Cytometry, User Optimized, human, mouse, rat, Xenopus laevis, Xenopus tropicalis, zebrafish, swine, bovine, chicken. Direct ELISA, 1:15, 000 - 1:30, 000, human, mouse, rat, Xenopus laevis, Xenopus tropicalis, zebrafish, swine, bovine, chicken. Immunohistochemistry: 1:500, human, mouse, rat, Xenopus laevis, Xenopus tropicalis, zebrafish, swine, bovine, chicken.
应用 :
免疫组化, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
Bovine, Chicken, Xenopus Laevis, Xenopus Tropicalis, Zebrafish
图像标记 :
SMAD3 was detected in paraffin-embedded sections of epidermal keratinocytes at 40X using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059) at 2.5 ug/mL. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA. Western blot analysis of endogenous SMAD3 expression in stimulated cell lysates. Lysates were prepared from control cells (- lanes), or cells stimulated with 2 ng/ml TGF for 1 hour. Phosphorylated SMAD3 was detected using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059). Personal Communication. Ying Zhang, NIH, CCR, Bethesda, MD. Western blot analysis of SMAD3 expression in HEK293 lysate (lane 1), HeLa Lysate (lane 2), MCF-7 Lysate (lane 3), Jurkat Lysate (lane 4), A431 Lysate (lane 5), A549 Lysate (lane 6), LNCap Lysate (lane 7), MOLT-4 Lysate (lane 8), Ramos Lysate (lane 9), Raji Lsyate (lane 10), A-172 Lysate (lane 11) and NIH/3T3 Lysate (lane 12). Phosphorylated SMAD3 at 35KD was detected using rabbit anti-SMAD3 phospho S423/phospho S425 Antigen Affinity purified polyclonal antibody (Catalog # P00059) at 1ug/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
背景 :
Mothers against decapentaplegic homolog 3 also known as SMAD family member 3 or SMAD3 is a protein that in humans is encoded by the SMAD3 gene. SMAD3 is a member of the SMAD family of proteins. It is located in 15q22.33. And it mediates the signals from the transforming growth factor beta (TGF-??) super family ligands that regulate cell proliferation, differentiation and death. Based on its essential role in TGF beta signaling pathway, SMAD3 has been related with tumor growth in cancer development.
competitor equivalent skus :
sc 101154
研究种类 :
Cancer, Cancer Metabolism, Cytoplasmic, Epigenetics And Nuclear Signaling, Metabolism, Metabolism Processes, Nuclear Signaling, Nuclear Signaling Pathways, Pathways And Processes, Response To Hypoxia, Signal Transduction, Signaling Pathway, Stem Cells, Tgf Beta
同义词 :
Mothers against decapentaplegic homolog 3;MAD homolog 3;Mad3;Mothers against DPP homolog 3;hMAD-3;JV15-2;SMAD family member 3;SMAD 3;Smad3;hSMAD3;SMAD3;MADH3;
基因全称 :
Mothers against decapentaplegic homolog 3
分子量 :
48081分子量
protein function :
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
subcellular localization :
Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta.
更新 :
1/28/19 19:39
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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