产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Phospho-Tau (T231) MAPT Rabbit Monoclonal Antibody
目录 :
P00097-1
规格 :
100微升
价格 :
315美元
克隆性 :
单克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
克隆名称 :
EIH-13
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 P00097-1 图像 1
Western blot analysis of Phospho-Tau (T231) expression in (1) SH-SY5Y cell lysate; (2) SH-SY5Y cell lysate, treated with sorbitol (P00097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPT monoclonal antibody (Catalog # P00097-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPT
产品信息
SKU号 :
P00097-1
产品名称 :
Anti-Phospho-Tau (T231) MAPT Rabbit Monoclonal Antibody
价格 :
315美元
规格 :
100微升
克隆性 :
单克隆
克隆编号 :
EIH-13
宿主 :
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
免疫沉淀, 免疫组化, 免疫印迹
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:200。 免疫沉淀1:50
描述 :
Boster Bio Anti-Phospho-Tau (T231) MAPT Rabbit Monoclonal Antibody catalog # P00097-1. Tested in IP, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
基因名称 :
MAPT
UniProt数据库编号 :
P10636
免疫原 :
A synthesized peptide derived from human Phospho-Tau (T231)
形式 :
液体
Contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA
纯化 :
亲和色谱
储存 :
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
基因全称 :
Microtubule-associated protein tau
同义词 :
Microtubule-associated protein tau; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; MAPT; MAPTL, MTBT1, TAU;
分子量 :
78928分子量
Protein Function :
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Subcellular Localization :
Cytoplasm, cytosol. Cell membrane ; Peripheral membrane protein ; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
组织特异性 :
Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
研究种类 :
Alzheimer'S Disease, Cell Type Marker, Cytoskeleton, Cytoskeleton / Ecm, Microtubules, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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