产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Phospho-Tau (T231) Rabbit Monoclonal Antibody
目录 :
P00097-1
规格 :
100 ug/vial
价格 :
299美元
克隆性 :
单克隆
宿主 :
共轭标签 :
nonconjugated
克隆名称 :
EIH-13
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫沉淀
图像
图像 1 :
武汉博士德生物工程有限公司 P00097-1 图像 1
Western blot analysis of Phospho-Tau (T231) expression in (1) SH-SY5Y cell lysate; (2) SH-SY5Y cell lysate, treated with sorbitol (P00097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPT monoclonal antibody (Catalog # P00097-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPT
产品信息
SKU号 :
P00097-1
状态 :
Enabled
名称 :
Anti-Phospho-Tau (T231) Rabbit Monoclonal Antibody
目录名称 :
一抗, 单克隆抗体, 兔单克隆抗体, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
MAPT
价格 :
299美元
price various sizes :
100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299 30ug sample size / $99
克隆性 :
单克隆
克隆编号 :
EIH-13
浓度 :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
共轭标签 :
No
contents :
兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油
描述 :
Monoclonal antibody for Tau/MAPT detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IP, IHC, WB. Reactive species: Human, Mouse, Rat Tau/MAPT information: Molecular Weight: 78928 MW; Subcellular Localization: Cytoplasm, cytosol . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell projection, axon . Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components; Tissue Specificity: Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
short description :
Anti-Phospho-Tau (T231) Rabbit Monoclonal Antibody tested for IP, IHC, WB in Human, Mouse, Rat
规格 :
100 ug/vial
sample size available :
30ul for $99, contact us for details
UniProt数据库编号 :
P10636
宿主 :
免疫原 :
A synthesized peptide derived from human Phospho-Tau (T231)
形式 :
Liquid
纯化 :
亲和色谱
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.
应用细节 :
免疫印迹1:500-1:2000。 免疫组化1:50-1:200。 免疫沉淀1:50
应用 :
免疫沉淀, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Western blot analysis of Phospho-Tau (T231) expression in (1) SH-SY5Y cell lysate; (2) SH-SY5Y cell lysate, treated with sorbitol (P00097-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPT monoclonal antibody (Catalog # P00097-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAPT
研究种类 :
Alzheimer'S Disease, Cell Type Marker, Cytoskeleton, Cytoskeleton / Ecm, Microtubules, Neurodegenerative Disease, Neurology Process, Neuron Marker, Neuroscience, Signal Transduction
同义词 :
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau;MAPT;MAPTL, MTBT1, TAU;
基因全称 :
Microtubule-associated protein tau
分子量 :
78928分子量
protein function :
Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
subcellular localization :
Cytoplasm, cytosol . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell projection, axon . Mostly found in the axons of neurons, in the cytosol and in association with plasma membrane components.
组织特异性 :
Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
更新 :
4/22/19 1:33
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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