抗体

抗磷酸化Raf1(S621)兔单克隆抗体

P00446-4

100微升

315美元

单克隆

domestic rabbit

未共轭

IOF-18

人类, 小鼠, 大鼠

抗磷酸化Raf1(S621)兔单克隆抗体

315美元

100微升

单克隆

IOF-18

兔

人类, 小鼠, 大鼠

免疫印迹

免疫印迹1:500-1:2000

Boster Bio Anti-Phospho-Raf1 (S621) Rabbit Monoclonal Antibody catalog # P00446-4. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

RAF1

P04049

A synthesized peptide derived from human Phospho-Raf1 (S621)

液体

兔IgG在磷酸盐缓冲液, pH值7.4, 150mM氯化钠, 0.02% 叠氮化钠和50% 甘油, 0.4 0.5毫克/毫升BSA

亲和色谱

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

RAF原癌基因丝氨酸/苏氨酸蛋白激酶

RAF proto-oncogene serine/threonine-protein kinase; 2.7.11.1; Proto-oncogene c-RAF; cRaf; Raf-1; RAF1; RAF;

73052分子量

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2- antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.

Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid- induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.

In skeletal muscle, isoform 1 is more abundant than isoform 2.

Apoptosis, Cancer, Cell Biology, Cell Death, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oncoproteins, Oncoproteins/Suppressors, Pathways And Processes, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction