产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗连接蛋白/GJA1抗体
目录 :
PA1026-1
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Figure 1. IHC analysis of Connexin 43/GJA1 using anti- Connexin 43/GJA1 antibody (PA1026-1). Connexin 43/GJA1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Connexin 43/GJA1 Antibody (PA1026-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 2 :
Figure 2. IHC analysis of Connexin 43/GJA1 using anti- Connexin 43/GJA1 antibody (PA1026-1). Connexin 43/GJA1 was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Connexin 43/GJA1 Antibody (PA1026-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
Figure 3. IHC analysis of Connexin 43/GJA1 using anti- Connexin 43/GJA1 antibody (PA1026-1). Connexin 43/GJA1 was detected in frozen section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Connexin 43/GJA1 Antibody (PA1026-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PA1026-1
产品名称 :
抗连接蛋白/GJA1抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
应用(s) :
免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
Immunocytochemistry , 0.5-1µg/mlImmunohistochemistry (Frozen Section), 0.5-1µg/mlImmunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml Western blot, 0.1-0.5µg/ml.
应用注释 :
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Connexin 43/GJA1 Antibody catalog # PA1026-1. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
GJA1
UniProt数据库编号 :
P17302
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43 (351-367aa HELQPLAIVDQRPSSRA), identical to the related rat and mouse sequences.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
间隙连接甲1蛋白
同义词 :
Gap junction alpha-1 protein; Connexin-43; Cx43; Gap junction 43 kDa heart protein; GJA1; GJAL;
蛋白名 :
间隙连接甲1蛋白
分子量 :
43008分子量
Protein Function :
Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity).
Subcellular Localization :
Cell membrane ; Multi-pass membrane protein. Cell junction, gap junction. Endoplasmic reticulum.
组织特异性 :
Expressed in the heart and fetal cochlea.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Sequence Similarities :
Belongs to the connexin family. Alpha-type (group II) subfamily.
背景 :
Connexins 43 (Cx43), also called GAP Junction Protein, alpha-1 (GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
研究种类 :
Atherosclerosis, Cardiovascular, Cell Adhesion, Cytoskeleton / Ecm, Signal Transduction, Vascular Inflammation
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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