抗体

抗HIF-1抗体

PA1041

100 ug/vial

200美元

多克隆

兔

nonconjugated

人类, 小鼠, 大鼠

免疫印迹, 免疫组化-石蜡切片

抗HIF-1抗体

200美元

A synthetic peptide corresponding to a sequence at the N-terminus of human HIF-1-alpha(8-21aa NDKKKISSERRKEK), different from the related rat and mouse sequences by two amino acids.

冻干

兔

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

免疫原亲和纯化

多克隆

Rabbit IgG polyclonal antibody for Hypoxia-inducible factor 1-alpha(HIF1A) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

100 ug/vial

人类;小鼠;大鼠

免疫组化-P;免疫印迹

HIF-1 alpha(Hypoxia-inducible factor 1 alpha,HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1 alpha transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1 alpha is over expressed during carcinogenesis, myocardial infarction and wound healing. It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers, resulting in the activation of genes essential for cell survival. HIF-1 alpha regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No cross reactivity with other proteins

缺氧诱导因子甲

缺氧诱导因子1A

鸡

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

缺氧诱导因子甲(HIF-1/HIF1甲)

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

cell biology apoptosis intracellular bcl2 family cardiovascular hypoxia hif microbiology interspecies interaction host virus interaction epigenetics and nuclear signaling transcription domain families hlh / leucine zipper hlh cell biology mitochondrial cardiovascular/immune cancer invasion/microenvironment heart cancer metabolism response to hypoxia metabolism pathways and processes mitochondrial metabolism mitochondrial markers metabolism processes types of disease cell death

Contains 1 bHLH (basic helix-loop-helix) domain.

At -20ËšC for one year. After reconstitution, at 4ËšC for one month. It can also be aliquotted and stored frozen at -20ËšC for a longer time.Avoid repeated freezing and thawing.

Cytoplasm. Nucleus. Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles (By similarity). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.

Hypoxia-inducible factor 1-alpha;HIF-1-alpha;HIF1-alpha;ARNT-interacting protein;Basic-helix-loop-helix-PAS protein MOP1;Class E basic helix-loop-helix protein 78;bHLHe78;Member of PAS protein 1;PAS domain-containing protein 8;HIF1A;BHLHE78, MOP1, PASD8;

Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

Q16665