抗体

Anti-Sonic Hedgehog/SHH Antibody

PA1072-1

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化

Anti-Sonic Hedgehog/SHH Antibody

315美元

100μg/vial

多克隆

兔

人类, 小鼠, 大鼠

仓鼠

免疫组化, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Sonic Hedgehog/SHH Antibody catalog # PA1072-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

SHH

Q15465

A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog (203-218aa ATVHLEQGGTKLVKDL), identical to the related rat and mouse sequences.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Sonic hedgehog protein

Sonic hedgehog protein; SHH; HHG-1; Sonic hedgehog protein N-product; Sonic hedgehog protein C-product; SHH;

Sonic hedgehog protein

49607分子量

Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior- posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity).

Sonic hedgehog protein C-product: Secreted, extracellular space. The C-terminal peptide diffuses from the cell.

Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Belongs to the hedgehog family.

The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.

Developmental Families, Domain Families, Embryogenesis, Embryonic Stem Cells, Epigenetics and Nuclear Signaling, Hypoxia, Intracellular, Metabolism, Metabolism Processes, Neural Stem Cells, Oncoproteins/Suppressors, Organogenesis, Pathways And Processes, Response To Hypoxia, Secreted, Signaling Pathways, Stem Cells, Surface Molecules, Transcription, Tumor Suppressors