图像 1 :
Anti- Topoisomerase II alpha antibody, PA1127, Western blotting. All lanes: Anti Topoisomerase II alpha (PA1127) at 0.5ug/ml. Lane 1: HELA Whole Cell Lysate at 40ug. Lane 2: JURKAT Whole Cell Lysate at 40ug. Predicted bind size: 174KD. Observed bind size: 174KD
免疫印迹, 0.1 0.5µg/毫升, 人类
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Boster Bio Anti-Topoisomerase II alpha/TOP2A Antibody catalog # PA1127. Tested in WB applications. This antibody reacts with Human.
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
A synthetic peptide corresponding to a sequence at the C-terminus of human Topoisomerase II alpha (1466-1480aa RKPSTSDDSDSNFEK), different from the related rat sequence by three amino acids, and different from the related mouse sequence by four amino acids
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
No cross reactivity with other proteins
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
DNA topoisomerase 2-alpha; 220.127.116.11; DNA topoisomerase II, alpha isozyme; TOP2A; TOP2;
Protein Function :
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).
Subcellular Localization :
Cytoplasm. Nucleus, nucleoplasm. Generally located in the nucleoplasm.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Sequence Similarities :
Belongs to the type II topoisomerase family.
The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22. The TOP2A gene spans approximately 30 kb and contains 35 exons. Furthermore, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes. Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, catenation, decatenation, knotting, and unknotting of circular DNA. It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments. There are about 100,000 molecules of topoisomerase II per HeLa cell nucleus, constituting about 0.1% of the nuclear extract. DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation. Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, but did not affect transcription on naked templates. Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II.
Cancer, Chromosome Structure, DNA / RNA, DNA Synthesis, Epigenetics and Nuclear Signaling
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