产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗CD34抗体
目录 :
PA1334
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, brown rat
应用 :
免疫印迹, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, 免疫组化-冰冻切片
文章摘录数: 2
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 人类; 1:200; 图 5i
Park M, Kim A, Manandhar S, Oh S, Jang G, Kang L, et al. CCN1 interlinks integrin and hippo pathway to autoregulate tip cell activity. elife. 2019;8: pubmed 出版商
Ayme Dietrich E, Lawson R, Cote F, de Tapia C, Da Silva S, Ebel C, et al. The role of 5-HT2B receptors in mitral valvulopathy: bone marrow mobilization of endothelial progenitors. Br J Pharmacol. 2017;174:4123-4139 pubmed 出版商
图像
图像 1 :
武汉博士德生物工程有限公司 PA1334 图像 1
Anti-CD34 antibody, PA1334, Western blotting. Lane 1: Recombinant Human CD34 Protein 10ng. Lane 2: Recombinant Human CD34 Protein 5ng. Lane 3: Recombinant Human CD34 Protein 2.5ng
图像 2 :
武汉博士德生物工程有限公司 PA1334 图像 2
Anti-CD34 antibody, PA1334, IHC(P). IHC(P): Rat Kidney Tissue
图像 3 :
武汉博士德生物工程有限公司 PA1334 图像 3
Anti-CD34 antibody, PA1334, IHC(P). IHC(P): Human Lung Cancer Tissue
产品信息
SKU号 :
PA1334
状态 :
Enabled
名称 :
抗CD34抗体
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
CD34
价格 :
280美元
price various sizes :
30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
描述 :
Polyclonal antibody for CD34 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB, IHC-P, IHC-F, ICC, FCM. Reactive species: Human;Mouse;Rat. CD34 information: Molecular Weight: 40716 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein; Tissue Specificity: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
short description :
Rabbit IgG polyclonal antibody for Hematopoietic progenitor cell antigen CD34(CD34) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P28906
宿主 :
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human CD34(366-382aa QATSRNGHSARQHVVAD), identical to the related mouse and rat sequences.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Western blot, 0.1-0.5ug/ml, Human, Mouse, Rat. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat. Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Mouse, Rat, Human. Immunocytochemistry, 0.5-1ug/ml, Human. Flow Cytometry, 1-3ug/1x10 6 cells, Human.
应用 :
流式细胞仪, 免疫组化-P, 免疫组化-F, 免疫细胞化学, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
图像标记 :
Anti-CD34 antibody, PA1334, Western blotting. Lane 1: Recombinant Human CD34 Protein 10ng. Lane 2: Recombinant Human CD34 Protein 5ng. Lane 3: Recombinant Human CD34 Protein 2.5ng Anti-CD34 antibody, PA1334, IHC(P). IHC(P): Rat Kidney Tissue Anti-CD34 antibody, PA1334, IHC(P). IHC(P): Human Lung Cancer Tissue Anti-CD34 antibody, PA1334, IHC(F). IHC(F): Rat Kidney Tissue Anti-CD34 antibody, PA1334, IHC(F). IHC(F): Rat Placenta Tissue Figure 6. Flow Cytometry analysis of A431 cells using anti- CD34 antibody (PA1334). Overlay histogram showing A431 cells stained with PA1334 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- CD34 Antibody (PA1334,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control. Figure 7. IHC analysis of CD34 using anti- CD34 antibody (PA1334). CD34 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CD34 Antibody (PA1334) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
背景 :
CD34 is a monomeric cell surface antigen with a molecular mass of approximately 110 KD.CD34 is expressed in humans in hematopoietic stem cells, vascular endothelium, and blasts from 30% of patients with acute myeloid and lymphocytic leukemia. The human CD34 gene spans 26 kb and has 8 exons, a structure quite similar to that of the murine gene. By Southern blot analysis of DNA from a panel of human x mouse somatic cell hybrids using a CD34 cDNA probe demonstrate that the gene for CD34 is located on human chromosome 1 in the 1q12----qter region. CD34 plays an important role in the formation of progenitor cells during both embryonic and adult hematopoiesis.
competitor equivalent skus :
sc 9095 sc 133082 sc 7045 sc 19621 sc 74499 sc 65261 sc 18917 sc 52478 sc 53511 sc 19587 sc 52312 sc 7324
研究种类 :
Cancer, Cardiogenesis, Cardiovascular, Cell Type Marker, Cell Type Markers, Developmental Biology, Hematopoietic Progenitors, Immunology, Lineage Markers, Lineage Specification, Mesenchymal Stem Cells, Neuron Marker, Neuroscience, Stem Cells, Surface Molecules, Tumor Biomarkers
同义词 :
造血祖细胞抗原CD34;CD34;CD34;
基因全称 :
造血祖细胞抗原CD34
分子量 :
40716分子量
protein function :
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
subcellular localization :
Membrane; Single-pass type I membrane protein.
组织特异性 :
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
蛋白名 :
造血祖细胞抗原CD34
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
sequence similarities :
Belongs to the CD34 family.
更新 :
4/15/19 8:33
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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