产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗CDC42抗体
目录 :
PA1366
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类,
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 PA1366 图像 1
Anti-CDC42 antibody, PA1366, Western blotting. Lane 1: Recombinant Human CDC42 Protein 10ng. Lane 2: Recombinant Human CDC42 Protein 5ng. Lane 3: Recombinant Human CDC42 Protein 2.5ng
图像 2 :
武汉博士德生物工程有限公司 PA1366 图像 2
Anti-CDC42 antibody, PA1366, IHC(P). IHC(P): Human Mammary Cancer Tissue
产品信息
SKU号 :
PA1366
产品名称 :
抗CDC42抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
牛, 人类, 小鼠, 大鼠
前体活性 :
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human, Bovine, Mouse, Rat.
应用注释 :
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-CDC42 Antibody catalog # PA1366. Tested in IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
CDC42
UniProt数据库编号 :
P60953
免疫原 :
A synthetic peptide corresponding to a sequence in the middle region of human CDC42 (121-138aa DDPSTIEKLAKNKQKPIT), identical to the related mouse and rat sequences.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Cell division control protein 42 homolog
同义词 :
Cell division control protein 42 homolog; G25K GTP-binding protein; CDC42;
蛋白名 :
Cell division control protein 42 homolog(Cdc42 homolog)
分子量 :
21259分子量
Protein Function :
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Plays a role in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. Mediates CDC42-dependent cell migration.
Subcellular Localization :
Cell membrane ; Lipid-anchor ; Cytoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Midbody. Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase. Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells. Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1. Colocalizes with NEK6 in the centrosome.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Sequence Similarities :
Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
背景 :
Cell division control protein 42 homolog also known as CDC42 is a protein involved in regulation of the cell cycle. In humans, CDC42 is encoded by the CDC42 gene. CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
研究种类 :
Cancer, Cell Biology, Cell Cycle, Cell Division, Cytokinesis, Epigenetics and Nuclear Signaling, G Protein Signaling, Kinases/Phosphatases, Protein Trafficking, Ras Family, Signal Transduction, Signaling Pathway, Small G Proteins, Vesicle Transport
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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