产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗鼠双微基因2抗体
目录 :
PA1378-1
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹
更多信息或购买 :
图像
图像 1 :
Figure 1. Western blot analysis of MDM2 using anti- MDM2 antibody (PA1378-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- MDM2 antigen affinity purified polyclonal antibody (Catalog # PA1378-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDM2 at approximately 90KD. The expected band size for MDM2 is at 55KD.
产品信息
SKU号 :
PA1378-1
产品名称 :
抗鼠双微基因2抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类
前体活性 :
兔
应用(s) :
免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升, 人类
应用注释 :
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-MDM2 Antibody catalog # PA1378-1. Tested in WB applications. This antibody reacts with Human.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
鼠双微基因2
UniProt数据库编号 :
Q00987
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human MDM2 (343-358aa EKAKLENSTQAEEGFD), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
E3泛素蛋白连接酶
鼠双微基因2
同义词 :
E3 ubiquitin-protein ligase Mdm2; 6.3.2.-; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2; MDM2;
蛋白名 :
E3泛素蛋白连接酶
鼠双微基因2
分子量 :
55233分子量
Protein Function :
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation.
Subcellular Localization :
Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF (P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
组织特异性 :
Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Sequence Similarities :
Belongs to the MDM2/MDM4 family.
背景 :
Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain (TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al. (1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.
研究种类 :
Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics and Nuclear Signaling, Oncoproteins, Oncoproteins/Suppressors, P53 Pathway, Transcription, Ubiquitin & Ubiquitin Like Modifiers
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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