抗体

抗鼠双微基因2抗体

PA1378

100 ug/vial

240美元

多克隆

兔

nonconjugated

人类, 小鼠, 大鼠

Enabled

抗鼠双微基因2抗体

一抗, 多克隆抗体

鼠双微基因2

240美元

30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240

多克隆

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Polyclonal antibody for HDM2/MDM2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. HDM2/MDM2 information: Molecular Weight: 55233 MW; Subcellular Localization: Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus; Tissue Specificity: Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.

Rabbit IgG polyclonal antibody for E3 ubiquitin-protein ligase Mdm2(MDM2) detection. Tested with WB in Human;Mouse;Rat.

100 ug/vial

30ug for $99, contact us for details

Q00987

兔

A synthetic peptide corresponding to a sequence at the N-terminus of human MDM2(103-119aa RKIYTMIYRNLVVVNQQ), different from the related mouse sequence by three amino acids, and different from rat sequence by two amino acids.

冻干

免疫原亲和纯化

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

免疫印迹, 0.1 0.5微克/毫升, 人类, 小鼠, 大鼠

免疫印迹

人类, 小鼠, 大鼠

鸡

Anti-MDM2 antibody, PA1378, Western blotting. Lane 1: Mouse Liver Tissue Lysate. Lane 2: Mouse Spleen Tissue Lysate. Lane 3: Mouse Brain Tissue Lysate. Lane 4: Mouse Thymus Tissue Lysate. Lane 5: Mouse Ovary Tissue Lysate.

Mdm2 is an important negative regulator of the p53 tumor suppressor. It is the name of a gene as well as the protein encoded by that gene. Mdm2 protein functions both as an E3 ubiquity lipase that recognizes the N-terminal trans-activation domain(TAD) of the p53 tumor suppressor and an inhibitor of p53 transcriptional activation. Oliner et al.(1992) used MDM2 clones to localize the human gene to chromosome 12q13-q14 by analysis of human-hamster somatic cell hybrids.

sc 56154 sc 56430 sc 965 sc 813 sc 7918 sc 5304 sc 812 sc 13161 sc 965 X

Cancer, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Epigenetics And Nuclear Signaling, Oncoproteins, Oncoproteins/Suppressors, P53 Pathway, Transcription, Ubiquitin & Ubiquitin Like Modifiers

E3 ubiquitin-protein ligase Mdm2;6.3.2.-;Double minute 2 protein;Hdm2;Oncoprotein Mdm2;p53-binding protein Mdm2;MDM2;

E3泛素蛋白连接酶 鼠双微基因2

55233分子量

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation.

Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.

Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.

E3泛素蛋白连接酶 鼠双微基因2

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Belongs to the MDM2/MDM4 family.

1/28/19 19:39