抗体

Anti-Fractalkine CX3CL1 Antibody

PA1401

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

小鼠, 大鼠

免疫印迹, 免疫组化

Anti-Fractalkine CX3CL1 Antibody

315美元

100μg/vial

多克隆

兔

小鼠, 大鼠

免疫组化, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Mouse, Rat, By Heat. Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Mouse, Rat Western blot, 0.1-0.5µg/ml, Mouse, Rat.

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Fractalkine CX3CL1 Antibody catalog # PA1401. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

CX3CL1

O35188

A synthetic peptide corresponding to a sequence at the N-terminus of mouse CX3CL1 (74-92aa CADPKEKWVQDAMKHLDHQ), identical to the related rat sequence.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Fractalkine

Fractalkine; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Neurotactin; Small-inducible cytokine D1; Processed fractalkine; Cx3cl1; Cx3c, Fkn, Scyd1;

Fractalkine

42099分子量

The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (By similarity). Binds to CX3CR1.

Cell membrane; Single-pass type I membrane protein.

Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).

Chemokine (C-X3-C motif) ligand 1, also known as CX3CL1, is a large cytokine protein. It is said to be the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Its gene is mapped to the long arm of chromosome 8. Being the large protein, it has got 373 amino acids. Imai et al. (1997) identified a 7-transmembrane high-affinity receptor (CX3CR1; 601470) for fractalkine and showed that it mediates both the adhesive and migratory functions of fractalkine.

Atherosclerosis, Cardiovascular, Chemokines, Immunology, Innate Immunity, Macrophage / Inflamm., Vascular Inflammation