产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗MAPK8/9抗体
目录 :
PA1407
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 PA1407 图像 1
Anti-MAPK8/9 antibody, PA1407, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Thymus Tissue Lysate. Lane 3: MCF-7 Cell Lysate. Lane 4: HELA Cell Lysate. Lane 5: JURKAT Cell Lysate. Lane 6: MM231 Cell Lysate. Lane 7: CEM Cell Lysate
图像 2 :
武汉博士德生物工程有限公司 PA1407 图像 2
Anti-MAPK8/9 antibody, PA1407, IHC(P). IHC(P): Rat Intestine Tissue
图像 3 :
武汉博士德生物工程有限公司 PA1407 图像 3
Anti-MAPK8/9 antibody, PA1407, IHC(P). IHC(P): Human Intestinal Cancer Tissue
产品信息
SKU号 :
PA1407
产品名称 :
抗MAPK8/9抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. - Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.
应用注释 :
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-MAPK8/9 Antibody catalog # PA1407. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
MAPK8
UniProt数据库编号 :
P45983, P45984
免疫原 :
A synthetic peptide corresponding to a sequence in the middle region of human MAPK8/9 (EREHTIEEWKELIYKEVMDL), identical to the related mouse and rat sequences.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
丝裂原活化蛋白激酶8/9
同义词 :
丝裂原活化蛋白激酶8;地图激酶8;MAPK 8;2.7。 11.24;JNK-46;压力激活的蛋白激酶1c;SAPK1c;应激活化蛋白激酶JNK1;c Jun氨基末端激酶1;MAPK8;JNK1, PRKM8, SAPK1, SAPK1C;JNK2, MAPK9
蛋白名 :
丝裂原活化蛋白激酶8/9(地图激酶8/MAPK 8/9)
分子量 :
48296分子量
Protein Function :
Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).
Subcellular Localization :
Cytoplasm. Nucleus.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Sequence Similarities :
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
背景 :
Mitogen-activated protein kinase 8 is an enzyme that in humans is encoded by the MAPK8 gene. The protein encoded by this gene is a member of the MAP kinase family. The International Radiation Hybrid Mapping Consortium mapped the MAPK8 gene to chromosome 10. The single long open reading frame encodes a putative protein kinase with a predicted mass of 44.2 kD. This kinase play a key role in T cell proliferation, apoptosis and differentiation. The protein encoded by MAPK9 gene is also a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.
研究种类 :
Cancer, Cancer Susceptibility, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Mapk Pathway, Oncoproteins, Oncoproteins/Suppressors, Protein Phosphorylation, Proto-Oncogenes, Ser / Thr Kinases, Serine/Threonine Kinases, Signal Transduction, Tlr Signaling, Transcription
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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