图像 1 :
Anti-Ubiquitin antibody, PA1420, Western blotting. Lane 1: Rat Thymus Tissue Lysate. Lane 2: Human MCF-7 Cell Lysate. Lane 3: MM231 Cell Lysate. Lane 4: HELA Cell Lysate. Lane 5: SMMC Cell Lysate .
图像 2 :
Anti-Ubiquitin antibody, PA1420, IHC(P). IHC(P): Human Lung Cancer Tissue
大鼠, 小鼠, 人类
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Boster Bio Anti-Ubiquitin/UBB Antibody catalog # PA1420. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
A synthetic peptide corresponding to a sequence at the N-terminus of human Ubiquitin (29-45aa KIQDKEGIPPDQQRLIF), identical to the rat and mouse sequences.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
No cross reactivity with other proteins
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Protein Function :
Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Subcellular Localization :
Ubiquitin: Cytoplasm. Nucleus.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Sequence Similarities :
Belongs to the ubiquitin family.
Ubiquitin (originally, ubiquitous immunopoietic polypeptide) is a small regulatory protein that has been found in almost all tissues (ubiquitously) of eukaryotic organisms. Its gene is mapped to 16p 13.3. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Ubiquitin binds to proteins and labels them for destruction. It directs protein recycling and also direct proteins to other locations in the cell, where they control other protein and cell mechanisms.
Alzheimer'S Disease, Cell Biology, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Neurodegenerative Disease, Neurology Process, Neuroscience, Proteasome / Ubiquitin, Proteolysis / Ubiquitin
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