抗体

抗胱氨酸-天冬氨酸蛋白酶7(P11)/CASP7抗体

PA1442

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化

抗胱氨酸-天冬氨酸蛋白酶7(P11)/CASP7抗体

315美元

100μg/vial

多克隆

兔

人类, 小鼠, 大鼠

仓鼠

免疫组化, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Caspase-7 (P11)/CASP7 Antibody catalog # PA1442. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

CASP7

P55210

A synthetic peptide corresponding to a sequence in the middle region of human Caspase-7 (P11) (237-255aa RGSWFVQALCSILEEHGKD), different from the related mouse and rat sequences by two amino acids.

冻干

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

胱氨酸-天冬氨酸蛋白酶7

Caspase-7; CASP-7; 3.4.22.60; Apoptotic protease Mch-3; CMH-1; ICE-like apoptotic protease 3; ICE-LAP3; Caspase-7 subunit p20; Caspase-7 subunit p11; CASP7; MCH3;

胱氨酸-天冬氨酸蛋白酶7(CASP-7)

34277分子量

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly- 217' bond. Overexpression promotes programmed cell death.

细胞质

Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

CASP7, Caspase-7, apoptosis-related cysteine peptidase, is a human protein encoded by the CASP7 gene. CASP7 orthologs have been identified in nearly all mammals for which complete genome data are available. CASP7 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis. Using radiation hybrid mapping, the CASP7 gene was localized to human chromosome 10q25.1-q25.2. The orderly activation of CASP7 regulates microglia activation through a protein kinase C-delta (PRKCD)-dependent pathway.

Apoptosis, Apoptotic Markers, Cancer, Caspases Etc, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways And Processes, Proteolysis / Ubiquitin, Proteolytic Enzymes