产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗MTA1抗体
目录 :
PA1483
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化-石蜡切片, 免疫组化-冰冻切片
图像
图像 1 :
武汉博士德生物工程有限公司 PA1483 图像 1
Anti-MTA1 antibody, PA1483, Western blotting. Lane 1: MCF-7 Cell Lysate . Lane 2: HELA Cell Lysate. Lane 3: JURKAT Cell Lysate . Lane 4: CEM Cell Lysate.
图像 2 :
武汉博士德生物工程有限公司 PA1483 图像 2
Anti-MTA1 antibody, PA1483, IHC(P). IHC(P): Human Rectal Cancer Tissue
图像 3 :
武汉博士德生物工程有限公司 PA1483 图像 3
Anti-MTA1 antibody, PA1483, IHC(F). IHC(F): Rat Ovary Tissue
产品信息
SKU号 :
PA1483
状态 :
Enabled
名称 :
抗MTA1抗体
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
MTA1
价格 :
280美元
price various sizes :
30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
描述 :
Polyclonal antibody for MTA1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. MTA1 information: Molecular Weight: 80786 MW; Subcellular Localization: Isoform Short: Cytoplasm; Tissue Specificity: Widely expressed. High expression in brain, liver, kidney, and cardiac muscle, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual. Up-regulated in a wide variety of cancers including breast, liver, ovarian, and colorectal cancer and its expression levels are closely correlated with tumor aggressiveness and metastasis.
short description :
Rabbit IgG polyclonal antibody for Metastasis-associated protein MTA1(MTA1) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
Q13330
宿主 :
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human MTA1(676-696aa ETKRAARRPYKPIALRQSQAL), identical to the related mouse and rat sequences.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Mouse, Rat, Human. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat. Western blot, 0.1-0.5ug/ml, Human, Rat, Mouse.
应用 :
免疫组化-P, 免疫组化-F, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
图像标记 :
Anti-MTA1 antibody, PA1483, Western blotting. Lane 1: MCF-7 Cell Lysate . Lane 2: HELA Cell Lysate. Lane 3: JURKAT Cell Lysate . Lane 4: CEM Cell Lysate. Anti-MTA1 antibody, PA1483, IHC(P). IHC(P): Human Rectal Cancer Tissue Anti-MTA1 antibody, PA1483, IHC(F). IHC(F): Rat Ovary Tissue Figure 4. IHC analysis of MTA1 using anti- MTA1 antibody (PA1483). MTA1 was detected in paraffin-embedded section of rat ovary tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- MTA1Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 5. IHC analysis of MTA1 using anti-MTA1 antibody (PA1483). MTA1 was detected in paraffin-embedded section of rat ovary tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 6. IHC analysis of MTA1 using anti-MTA1 antibody (PA1483). MTA1 was detected in frozen section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MTA1 Antibody (PA1483) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
背景 :
Metastasis-associated protein MTA1 is a protein that in humans is encoded by the MTA1 gene. This gene encodes a protein that was identified in a screen for genes expressed in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this gene has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues.By fluorescence in situ hybridization, mapped the MTA1gene to chromosome 14q32.3. MTA1 is a component of the chromatin remodeling complex that influences gene transcription by modulating target gene chromatin. MTA1 is widely upregulated in many carcinomas.
competitor equivalent skus :
sc 10813 sc 26653 sc 373765 sc 26654 sc 9446 sc 9445 sc 17773 sc 10813 X sc 17773 X
研究种类 :
Acetylation, Chromatin Modifying Enzymes, Epigenetics And Nuclear Signaling
同义词 :
Metastasis-associated protein MTA1;MTA1;
基因全称 :
Metastasis-associated protein MTA1
分子量 :
80786分子量
protein function :
Transcriptional coregulator which can act as both a transcriptional corepressor and coactivator. As a part of the histone-deacetylase multiprotein complex (NuRD), regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA. In conjunction with other components of NuRD, acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A. Acts as a transcriptional coactivator of BCAS3, PAX5 and SUMO2, independent of the NuRD complex. Stimulates the expression of WNT1 by inhibiting the expression of its transcriptional corepressor SIX3. Plays a role in the inflammatory responses, both as a target and as a component of the NF-kappa-B signaling and regulates a subset of proinflammatory cytokines such as IL1B, MIP2, and TNF. Regulates p53-dependent and -independent DNA repair processes following genotoxic stress. Regulates the stability and function of p53/TP53 by inhibiting its ubiquitination by COP1 and MDM2 thereby regulating the p53-dependent DNA repair. Plays an important role in tumorigenesis, tumor invasion, and metastasis. Involved in the epigenetic regulation of ESR1 expression in breast cancer in a TFAP2C, IFI16 and HDAC4/5/6-dependent manner. Plays a role in the regulation of the circadian clock and is essential for the generation and maintenance of circadian rhythms under constant light and for normal entrainment of behavior to light-dark (LD) cycles. Positively regulates the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of its own transcription and the transcription of CRY1. Regulates deacetylation of ARNTL/BMAL1 by regulating SIRT1 expression, resulting in derepressing CRY1- mediated transcription repression. Isoform Short binds to ESR1 and sequesters it in the cytoplasm and enhances its non-genomic responses.
subcellular localization :
Isoform Short: Cytoplasm.
组织特异性 :
Widely expressed. High expression in brain, liver, kidney, and cardiac muscle, ovaries, adrenal glands and virgin mammary glands. Higher in tumors than in adjacent normal tissue from the same individual. Up-regulated in a wide variety of cancers including breast, liver, ovarian, and colorectal cancer and its expression levels are closely correlated with tumor aggressiveness and metastasis.
蛋白名 :
Metastasis-associated protein MTA1
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
sequence similarities :
含1 BAH区域
更新 :
4/16/19 9:10
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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