产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗水通道蛋白3/AQP3抗体
目录 :
PA1488
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪
图像
图像 1 :
武汉博士德生物工程有限公司 PA1488 图像 1
Figure 1. Western blot analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Kidney Tissue Lysate. Lane 2: Rat Lung Tissue Lysate. Lane 3: Mouse Kidney Tissue Lysate. Lane 4: MM453 Whole Cell Lysate. Lane 5: SMMC-7721 Whole Cell Lysate . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Aquaporin 3 antigen affinity purified polyclonal antibody (Catalog # PA1488) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 3 at approximately 32KD. The expected band size for Aquaporin 3 is at 32KD.
图像 2 :
武汉博士德生物工程有限公司 PA1488 图像 2
Figure 2. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Aquaporin 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (PA1488) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
武汉博士德生物工程有限公司 PA1488 图像 3
Figure 3. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Aquaporin 3 was detected in immunocytochemical section of HELA cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (PA1488) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PA1488
状态 :
Enabled
名称 :
抗水通道蛋白3/AQP3抗体
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
AQP3
价格 :
280美元
price various sizes :
30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
描述 :
Polyclonal antibody for AQUAPORIN 3/AQP3 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. AQUAPORIN 3/AQP3 information: Molecular Weight: 31544 MW; Subcellular Localization: Basolateral cell membrane; Multi-pass membrane protein. In collecting ducts of kidney; Tissue Specificity: Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
short description :
Rabbit IgG polyclonal antibody for Aquaporin-3(AQP3) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
Q92482
宿主 :
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 3(278-292aa EENVKLAHVKHKEQI), different from the related rat and mouse sequences by one amino acid.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Rat, Mouse, By Heat . Immunocytochemistry , 0.5-1ug/ml, Human, Mouse, Rat. Immunohistochemistry(Frozen Section), 0.5-1ug/ml, Rat, Human, Mouse. Western blot, 0.1-0.5ug/ml, Human, Mouse, Rat. Flow Cytometry, 1-3ug/1x10 6 cells, Human
应用 :
流式细胞仪, 免疫组化, 免疫细胞化学, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
图像标记 :
Figure 1. Western blot analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Kidney Tissue Lysate. Lane 2: Rat Lung Tissue Lysate. Lane 3: Mouse Kidney Tissue Lysate. Lane 4: MM453 Whole Cell Lysate. Lane 5: SMMC-7721 Whole Cell Lysate . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Aquaporin 3 antigen affinity purified polyclonal antibody (Catalog # PA1488) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 3 at approximately 32KD. The expected band size for Aquaporin 3 is at 32KD. Figure 2. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Aquaporin 3 was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (PA1488) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 3. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Aquaporin 3 was detected in immunocytochemical section of HELA cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (PA1488) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 4. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Aquaporin 3 was detected in frozen section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (PA1488) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 5. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Aquaporin 3 was detected in paraffin-embedded section of human renal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (PA1488) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 6. IHC analysis of Aquaporin 3 using anti- Aquaporin 3 antibody (PA1488). Aquaporin 3 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Aquaporin 3 Antibody (PA1488) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 7. Flow Cytometry analysis of Hela cells using anti-Aquaporin 3 antibody (PA1488). Overlay histogram showing Hela cells stained with PA1488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Aquaporin 3 Antibody (PA1488,1ug/1x10 6 cells) for 30 min at 20°C. DyLight 488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
背景 :
This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
competitor equivalent skus :
sc 20811 sc 9885
研究种类 :
Cancer, Channels, Metabolism, Plasma Membrane, Signal Transduction
同义词 :
Aquaporin-3;AQP-3;Aquaglyceroporin-3;AQP3;
基因全称 :
水通道蛋白3
分子量 :
31544分子量
protein function :
Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity).
subcellular localization :
Basolateral cell membrane; Multi-pass membrane protein. In collecting ducts of kidney.
组织特异性 :
Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
蛋白名 :
水通道蛋白3
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
sequence similarities :
Belongs to the MIP/aquaporin (TC 1.A.8) family.
更新 :
1/28/19 19:39
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits