产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗MEKK2/MAP3K2抗体
目录 :
PA1499
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹
更多信息或购买 :
图像
图像 1 :
Figure 1. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. Lane 2: human K562 whole cell lysates. Lane 3: human THP-1 whole cell lysates. Lane 4: human Raji whole cell lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
图像 2 :
Figure 2. Western blot analysis of MEKK2 using anti-MEKK2 antibody (PA1499). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates. Lane 2: rat brain tissue lysates. Lane 3: rat heart tissue lysates. Lane 4: rat C6 whole cell lysates. Lane 5: mouse kidney tissue lysates. Lane 6: mouse brain tissue lysates. Lane 7: mouse heart tissue lysates. Lane 8: mouse Neuro-2a whole cell lysates. Lane 9: mouse NIH/3T3 whole cell lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK2 antigen affinity purified polyclonal antibody (Catalog # PA1499) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK2 at approximately 78KD. The expected band size for MEKK2 is at 70KD.
产品信息
SKU号 :
PA1499
产品名称 :
抗MEKK2/MAP3K2抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
Canine, Horse, Monkey, Rabbit
应用(s) :
免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升, 人类, 大鼠, 小鼠
应用注释 :
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-MEKK2/MAP3K2 Antibody catalog # PA1499. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
MAP3K2
UniProt数据库编号 :
Q9Y2U5
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK2 (606-619aa SADELLRHMFVHYH), different from the related rat and mouse sequences by one amino acid.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
丝裂原活化蛋白激酶激酶激酶
2
同义词 :
丝裂原活化蛋白激酶激酶激酶
2;2.7。 11.25;MAPK/ERK激酶激酶2;丁酮激酶;MEKK 2;MAP3K2;MAPKKK2, MEKK2;
蛋白名 :
丝裂原活化蛋白激酶激酶激酶
2
分子量 :
69741分子量
Protein Function :
Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics.
Subcellular Localization :
Cytoplasm. Nucleus. Upon EGF stimulation, translocates into the nucleus.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Sequence Similarities :
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
背景 :
MAP3K2, mitogen-activated protein kinase kinase kinase 2, also known as MEKK2, belongs to the mitogen-activated protein kinase (MAPK) kinase kinase gene family, which is involved in regulating multiple MAPK signaling pathways. Endogenous MAP3K2 localized mainly to the cytosol of unstimulated HeLa cells and rat fibroblasts. The MAP3K2 gene is mapped to chromosome 2 by the International Radiation Hybrid Mapping Consortium. MAP3K2 is a major upstream activator of the JNK, MAPK cascade. MAP3K2 was identifies as 1 of 6 kinases that regulate different steps of the caveolar cycle. Silencing of MAP3K2 strongly reduced kiss-and-run dynamics, leading to an accumulation of caveolar structures at the cell surface without affecting caveolae coat assembly or clustering.
研究种类 :
Mapk Pathway, Protein Phosphorylation, Ser / Thr Kinases, Signal Transduction
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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