产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗BOB1/POU2AF1抗体
目录 :
PA1506
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
图像
图像 1 :
武汉博士德生物工程有限公司 PA1506 图像 1
Anti-BOB1 antibody, PA1506, Western blotting. Lane: Rat Spleen Tissue Lysate
图像 2 :
武汉博士德生物工程有限公司 PA1506 图像 2
Anti-BOB1 antibody, PA1506, IHC(P). IHC(P): Rat Tonsil Tissue
产品信息
SKU号 :
PA1506
产品名称 :
抗BOB1/POU2AF1抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
大鼠, 小鼠
前体活性 :
仓鼠
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Mouse.
应用注释 :
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-BOB1/POU2AF1 Antibody catalog # PA1506. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
POU2AF1
UniProt数据库编号 :
Q64693
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1 (238-256aa EEEESNTYELNHTLSVEGF), different from the related rat sequence by one amino acid, and from human sequence by four amino acids.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
POU domain class 2-associating factor 1
同义词 :
POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; BOB1; OCA-B; OCT-binding factor 1; Pou2af1; Obf-1;
蛋白名 :
POU domain class 2-associating factor 1
分子量 :
27692分子量
Protein Function :
Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Subcellular Localization :
Nucleus.
组织特异性 :
B-cell specific.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
背景 :
POU2AF1 (POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al. (2002) reported that OCAB is essential for V (D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al. (2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.
研究种类 :
Adaptive Immunity, B Cells, Developmental Families, Domain Families, Epigenetics and Nuclear Signaling, Immunology, Non-Cd, Transcription
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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