产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Macrosialin CD68 Antibody
目录 :
PA1518
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
小鼠, 大鼠
应用 :
免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片, 免疫组化-冰冻切片
更多信息或购买 :
文章摘录数: 1
图像
图像 1 :
Figure 1. Western blot analysis of CD68 using anti- CD68 antibody (PA1518). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Spleen Tissue Lysate. Lane 2: Mouse Spleen Tissue Lysate. Lane 3: Mouse RAW246.7 Tissue Lysate . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CD68 antigen affinity purified polyclonal antibody (Catalog # PA1518) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
图像 2 :
Figure 2. IHC analysis of CD68 using anti- CD68 antibody (PA1518). CD68 was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CD68 Antibody (PA1518) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
Figure 3. IHC analysis of CD68 using anti- CD68 antibody (PA1518). CD68 was detected in paraffin-embedded section of Rat Spleen tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- CD68 Antibody (PA1518) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PA1518
产品名称 :
Anti-Macrosialin CD68 Antibody
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
小鼠, 大鼠
前体活性 :
仓鼠
应用(s) :
IF, 免疫组化-P, 免疫组化-F, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml, Rat, Mouse.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Mouse, Rat, By Heat.
Immunohistochemistry (Frozen Section), 0.5-1µg/ml, Mouse, Rat.
Immunofluorescence, 2μg/ml, Rat.
应用注释 :
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Macrosialin CD68 Antibody catalog # PA1518. Tested in IF, IHC-P, IHC-F, WB applications. This antibody reacts with Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
CD68
UniProt数据库编号 :
P31996
免疫原 :
A synthetic peptide corresponding to a sequence in the middle region of mouse CD68 (312-326aa AFCITRRRQSTYQPL), different from the related rat sequence by one amino acid.
形式 :
冻干
Contents :
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
基因全称 :
Macrosialin
同义词 :
Macrosialin; CD68; Cd68;
蛋白名 :
Macrosialin
分子量 :
34818分子量
Protein Function :
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Subcellular Localization :
Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein.
组织特异性 :
Expressed in tissue macrophages and to a lesser extent in dendritic cells.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
Sequence Similarities :
Belongs to the LAMP family.
背景 :
CD68 is a heavily glycosylated glycoprotein that is highly expressed in macrophages and other mononuclear phagocytes. Macrosialin, if not glycosylated, has the main sequence that consists of 354 amino acids with an expected molecular weight of 37.4 kD. CD68, which can be located in the cytoplasmic granules of a variety of blood cells and myocytes, can be detected via immunohistochemistry. It's particularly effective as a marker for monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts, all of which belong to the macrophage lineage. CD68 is expressed in tissue macrophages and to a lesser extent in dendritic cells.
CD68 is involved in tissue macrophage phagocytic activities, as well as intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It works by binding to tissue and organ-specific lectins or selectins, enabling macrophage subsets to be directed to specific locations. Macrophages may be able to crawl over selectin-bearing substrates or other cells if CD68 is rapidly recirculated from endosomes and lysosomes to the plasma membrane.
研究种类 :
细胞类型标记, 免疫学
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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