抗体

抗BCAT1抗体

PA1538

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

免疫印迹, 免疫组化-石蜡切片

抗BCAT1抗体

315美元

100μg/vial

多克隆

兔

人类

Monkey, Rabbit

免疫组化-P, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human.

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-BCAT1 Antibody catalog # PA1538. Tested in IHC-P, WB applications. This antibody reacts with Human.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

BCAT1

P54687

A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT1 (360-375aa KLASRILSKLTDIQYGR), different from the related mouse sequence by one amino acid, rat sequence by three amino acids.

冻干

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Branched-chain-amino-acid aminotransferase, cytosolic

Branched-chain-amino-acid aminotransferase, cytosolic; BCAT (c); 2.6.1.42; Protein ECA39; BCAT1; BCT1, ECA39;

Branched-chain-amino-acid aminotransferase, cytosolic(BCAT(c))

42966分子量

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

细胞质

During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.

Amino Acid Metabolism, Amino Acids, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Signal Transduction