产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗GRB2抗体
目录 :
PA1579
规格 :
100 ug/vial
价格 :
240美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
图像
图像 1 :
武汉博士德生物工程有限公司 PA1579 图像 1
Figure 1. Western blot analysis of GRB2 using anti-GRB2 antibody (PA1579). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. Lane 2: rat brain tissue lysates. Lane 3: rat spleen tissue lysates. Lane 4: mouse spleen tissue lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB2 antigen affinity purified polyclonal antibody (Catalog # PA1579) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB2 at approximately 25KD. The expected band size for GRB2 is at 25KD.
产品信息
SKU号 :
PA1579
状态 :
Enabled
名称 :
抗GRB2抗体
目录名称 :
一抗, 多克隆抗体
基因名称 :
GRB2
价格 :
240美元
price various sizes :
30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
描述 :
Polyclonal antibody for GRB2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. GRB2 information: Molecular Weight: 25206 MW; Subcellular Localization: Nucleus . Cytoplasm . Endosome . Golgi apparatus .
short description :
Rabbit IgG polyclonal antibody for Growth factor receptor-bound protein 2(GRB2) detection. Tested with WB in Human;Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P62993
宿主 :
兔
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human GRB2(184-201aa HVMDNSDPNWWKGACHGQ), identical to the related rat and mouse sequences.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
免疫印迹, 0.1 0.5微克/毫升, 人类, 大鼠, 小鼠
应用 :
免疫印迹
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
牛
图像标记 :
Figure 1. Western blot analysis of GRB2 using anti-GRB2 antibody (PA1579). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. Lane 2: rat brain tissue lysates. Lane 3: rat spleen tissue lysates. Lane 4: mouse spleen tissue lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB2 antigen affinity purified polyclonal antibody (Catalog # PA1579) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB2 at approximately 25KD. The expected band size for GRB2 is at 25KD.
背景 :
GRB2, Growth factor receptor-bound protein 2, is an adaptor protein involved in signal transduction/cell communication. The GRB2 gene encodes a protein that has homology to noncatalytic regions of the SRC oncogene product. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. In humans, the GRB2 protein is encoded by the GRB2 gene. Grb2 is widely expressed and is essential for multiple cellular functions. The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
competitor equivalent skus :
sc 137056 sc 13953 sc 28839 sc 137074 sc 17813 sc 8034 sc 365619 sc 255 sc 503 sc 255 G
研究种类 :
Adapters, Cancer, Cytoplasmic, G Protein Signaling, Ras Family, Signal Transduction, Small G Proteins
同义词 :
Growth factor receptor-bound protein 2;Adapter protein GRB2;Protein Ash;SH2/SH3 adapter GRB2;GRB2;ASH;
基因全称 :
Growth factor receptor-bound protein 2
分子量 :
25206分子量
protein function :
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
subcellular localization :
Nucleus . Cytoplasm . Endosome . Golgi apparatus .
蛋白名 :
Growth factor receptor-bound protein 2
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
sequence similarities :
Belongs to the GRB2/sem-5/DRK family.
更新 :
5/5/19 1:38
更多信息或购买 :
武汉博士德生物工程有限公司 产品网页
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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