产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗整合素乙3/ITGB3抗体
目录 :
PA1627
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 流式细胞仪, 免疫组化-石蜡切片
更多信息或购买 :
图像
图像 1 :
Anti-Integrin beta 3 antibody, PA1627, Western blotting. Lane 1: JURKAT Cell Lysate. Lane 2: RAJI Cell Lysate. Lane 3: CEM Cell Lysate. Lane 4: COLO320 Cell Lysate.
图像 2 :
Anti-Integrin beta 3 antibody, PA1627, IHC(P). IHC(P): Human Mammary Cancer Tissue
图像 3 :
Figure 3. Flow Cytometry analysis of H-PBMC cells using anti-Integrin beta 3 antibody (PA1627). Overlay histogram showing H-PBMC cells stained with PA1627 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin beta 3 Antibody (PA1627, 1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
PA1627
产品名称 :
抗整合素乙3/ITGB3抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
应用(s) :
流式细胞仪, 免疫组化-P, 免疫印迹
应用细节 :
Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Mouse, Rat, By Heat.
Flow Cytometry, 1-3µg/1x10^6 cells, Human.
应用注释 :
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Integrin beta 3/ITGB3 Antibody catalog # PA1627. Tested in Flow Cytometry, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
ITGB3
UniProt数据库编号 :
P05106
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human Integrin beta 3 (752-770aa EFAKFEEERARAKWDTANN), identical to the related rat and mouse sequences.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
整合素乙3
同义词 :
整合素乙3;血小板膜糖蛋白IIIa;糖蛋白Ⅲa;CD61;ITGB3;GP3A;
蛋白名 :
整合素乙3
分子量 :
87058分子量
Protein Function :
Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha- IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Subcellular Localization :
Cell membrane ; Single- pass type I membrane protein. Cell projection, lamellipodium membrane. Cell junction, focal adhesion.
组织特异性 :
Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Sequence Similarities :
Belongs to the integrin beta chain family.
背景 :
ITGB3 (INTEGRIN, BETA-3), also called GP3A, GPIIIa, CD61, is a protein that in humans is encoded by the ITGB3 gene. GP3A is a cluster of differentiation found on thrombocytes. The ITGB3 complex belongs to the integrin class of cell adhesion molecule receptors that share a common heterodimeric structure with alpha and beta subunits. The GP3A gene is mapped to 17q21.32. And the GP3A gene has 14 exons. The 3-prime exon is larger than 1,700 nucleotides and contains the 3-prime untranslated region. The ITGB3 complex mediates platelet aggregation by acting as a receptor for fibrinogen. Although the ITGB3 is expressed on the cell surface at normal levels and is capable of function following extracellular stimulation, it could not be activated via the "inside-out" signaling pathways.
研究种类 :
Cell Adhesion, Cell Cycle, Chromosome Structure, Co-Activators/Co-Repressors, Cytoskeleton / Ecm, Epigenetics and Nuclear Signaling, Integrins, Nfkb Pathway, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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