抗体

抗肌球蛋白磷酸酶/PPP1R12A抗体

PA1681

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

人类, 小鼠

免疫印迹, 免疫细胞化学, 流式细胞仪

抗肌球蛋白磷酸酶/PPP1R12A抗体

315美元

100μg/vial

多克隆

兔

人类, 小鼠, 大鼠

鸡

流式细胞仪, IF, 免疫细胞化学, 免疫印迹

免疫印迹, 0.1 0.5µg/毫升, 人类, 小鼠, 大鼠。 免疫细胞化学/免疫荧光, 2µg/毫升, 人类。 流式细胞仪, 1-3μg/1x10^6细胞, 人类

Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Myosin Phosphatase/PPP1R12A Antibody catalog # PA1681. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

PPP1R12A

O14974

A synthetic peptide corresponding to a sequence at the N-terminus of human PPP1R12A (1-17aa MKMADAKQKRNEQLKRW), identical to the related rat and mouse sequences.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Protein phosphatase 1 regulatory subunit 12A

Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit; PPP1R12A; MBS, MYPT1;

Protein phosphatase 1 regulatory subunit 12A

115281分子量

Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN- dependent suppression of HIF1A activity.

Cytoplasm. Along actomyosin filaments and stress fibers.

Expressed in striated muscles, specifically in type 2a fibers (at protein level).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for ICC.

Contains 6 ANK repeats.

PPP1R12A (Protein phosphatase 1 regulatory subunit 12A), also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. PPP1R12A is one of the subunits of myosin phosphatase. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. The PPP1R12A gene is mapped on 12q21.2-q21.3. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in 2 ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.

Actin Assembly, Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Motor Proteins, Myosin, Signal Transduction