产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗TRPC6抗体
目录 :
PA1754
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化
更多信息或购买 :
图像
图像 1 :
Figure 1. Western blot analysis of TRPC6 using anti- TRPC6 antibody (PA1754). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Lung Tissue Lysate. Lane 2: 293T Cell Lysate. Lane 3: 293T Cell Lysate . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- TRPC6 antigen affinity purified polyclonal antibody (Catalog # PA1754) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106-120KD. The expected band size for TRPC6 is at 106KD.
图像 2 :
Figure 2. IHC analysis of TRPC6 using anti- TRPC6 antibody (PA1754). TRPC6 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- TRPC6 Antibody (PA1754) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
图像 3 :
Figure 3. IHC analysis of TRPC6 using anti- TRPC6 antibody (PA1754). TRPC6 was detected in paraffin-embedded section of Rat Brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- TRPC6 Antibody (PA1754) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PA1754
产品名称 :
抗TRPC6抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
Monkey
应用(s) :
免疫组化, 免疫印迹
应用细节 :
Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.
应用注释 :
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-TRPC6 Antibody catalog # PA1754. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
TRPC6
UniProt数据库编号 :
Q9Y210
免疫原 :
A synthetic peptide corresponding to a sequence in the middle region of human TRPC6 (249-265aa HDYFCKCNDCNQKQKHD), different from the related rat and mouse sequences by three amino acids.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Short transient receptor potential channel 6
同义词 :
Short transient receptor potential channel 6; TrpC6; Transient receptor protein 6; TRP-6; TRPC6; TRP6;
蛋白名 :
Short transient receptor potential channel 6(TrpC6)
分子量 :
106326分子量
Protein Function :
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
Subcellular Localization :
Membrane ; Multi-pass membrane protein.
组织特异性 :
Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Sequence Similarities :
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
背景 :
Transient receptor potential cation channel, subfamily C, member 6, also known as TRPC6, is a human gene encoding a protein of the same name. The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2).
研究种类 :
Calcium Channels, Calcium Signaling, Signal Transduction, Signaling Pathway
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
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