产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-GRP78 BiP/HSPA5 Antibody
目录 :
PA1815
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
图像
图像 1 :
武汉博士德生物工程有限公司 PA1815 图像 1
Anti-GRP78 BiP antibody, PA1815, Western blotting. Lane 1: Rat Testis Tissue Lysate. Lane 2: A549 Cell Lysate. Lane 3: MCF-7 Cell Lysate. Lane 4: HELA Cell Lysate.
图像 2 :
武汉博士德生物工程有限公司 PA1815 图像 2
Anti-GRP78 BiP antibody, PA1815, IHC(P). IHC(P): Rat Intestine Tissue
图像 3 :
武汉博士德生物工程有限公司 PA1815 图像 3
Anti-GRP78 BiP antibody, PA1815, IHC(P). IHC(P): Rat Cerebellum Tissue
产品信息
SKU号 :
PA1815
产品名称 :
Anti-GRP78 BiP/HSPA5 Antibody
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
大鼠, 小鼠, 人类
前体活性 :
应用(s) :
免疫组化, 免疫细胞化学, 免疫印迹
应用细节 :
Immunocytochemistry , 0.5-1µg/ml, Human, Mouse, RatImmunohistochemistry (Frozen Section), 0.5-1µg/ml, Rat, Human, MouseImmunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5µg/ml, Human, Rat, Mouse.
应用注释 :
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-GRP78 BiP/HSPA5 Antibody catalog # PA1815. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
HSPA5
UniProt数据库编号 :
P11021
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human GRP78 BiP (603-617aa EWLESHQDADIEDFK), identical to the related mouse and rat sequences.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
78 kDa glucose-regulated protein
同义词 :
78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca (2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; HSPA5; GRP78;
蛋白名 :
78 kDa glucose-regulated protein
分子量 :
72333分子量
Protein Function :
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Subcellular Localization :
Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Sequence Similarities :
Belongs to the heat shock protein 70 family.
背景 :
HSPA5 (heat shock 70kDa protein 5) also known as glucose-regulated protein, 78kD (GRP78) or BiP, is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. The HSPA5 gene is mapped on 9q33.3. Shen et al. (2002) concluded that HSPA5 retains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation of HSPA5 during ER stress allows ATF6 to be transported to the Golgi. The findings of Shen et al. (2002) demonstrated that HSPA5 is a key element in sensing the folding capacity within the ER.
研究种类 :
标签&细胞标记
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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