产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗STAT5a抗体
目录 :
PA1840
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 流式细胞仪
更多信息或购买 :
图像
图像 1 :
Anti-STAT5a antibody, PA1840, Western blotting. Lane 1: HELA Cell Lysate. Lane 2: COLO320 Cell Lysate. Lane 3: JURKAT Cell Lysate. Lane 4: CEM Cell Lysate.
图像 2 :
Figure 2. Flow Cytometry analysis of U20S cells using anti-STAT5A antibody (PA1840). Overlay histogram showing U20S cells stained with PA1840 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- STAT5A Antibody (PA1840,1ug/1x10 6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
图像 3 :
Figure 3. Flow Cytometry analysis of Jurkat cells using anti-STAT5A antibody (PA1840). Overlay histogram showing Jurkat cells stained with PA1840 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti- STAT5A Antibody (PA1840,1ug/1x10 6 cells) for 30 min at 20°C. DyLight?488 conjugated goat anti-rabbit IgG (BA1127, 5-10ug/1x10 6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x10 6 ) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
产品信息
SKU号 :
PA1840
产品名称 :
抗STAT5a抗体
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
兔
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
牛
应用(s) :
流式细胞仪, 免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升, 人类, 小鼠, 大鼠。 流式细胞仪, 1-3µg/1x10^6细胞, 人类
应用注释 :
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-STAT5a Antibody catalog # PA1840. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
STAT5A
UniProt数据库编号 :
P42229
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human STAT5a (735-751aa YNMYPQNPDHVLDQDGE).
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Signal transducer and activator of transcription 5A
同义词 :
Signal transducer and activator of transcription 5A; STAT5A; STAT5;
蛋白名 :
Signal transducer and activator of transcription 5A
分子量 :
90647分子量
Protein Function :
Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Subcellular Localization :
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Sequence Similarities :
Belongs to the transcription factor STAT family.
背景 :
STAT5 (Signal transducer and activator of transcription 5) also known as STAT5A, MGF, is a protein that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides. Hou et al. (1995) cloned the human STAT5 cDNA from an umbilical vein endothelial cell library and found that it encodes a 794-amino acid polypeptide with a predicted mass of approximately 90.5 kD. To analyze the possible role of STAT5 in human GH-induced proliferation, Friedrichsen et al. (2001) expressed a dominant-negative STAT5 mutant, STAT5A-delta-749, in INS-1 cells under the control of a doxycycline-inducible promoter by stable transfection. Chromatin immunoprecipitation analysis confirmed direct binding of STAT5 to the HIF2A promoter 344 bp upstream of the HIF2A start site. STAT5 overexpression independently resulted in erythroid commitment in megakaryocytic-erythroid progenitors, which was abrogated by knockdown of GATA1. STAT5 is responsible for the immediate-early induction of the long isoform of the BCL2-related protein in erythroid cells through direct binding to the promoter of the BCLX gene.
研究种类 :
Epigenetics and Nuclear Signaling, Nuclear Signaling, Signal Transduction, Signaling Pathway, Transcription
更多信息或购买 :
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits