产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-B Raf/BRAF Antibody
目录 :
PA1848
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 PA1848 图像 1
Anti-B Raf antibody, PA1848, Western blotting. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Testis Tissue Lysate. Lane 3: Rat Liver Tissue Lysate. Lane 4: SW620 Cell Lysate. Lane 5: COLO320 Cell Lysate. Lane 6: PC-12 Cell Lysate.
产品信息
SKU号 :
PA1848
产品名称 :
Anti-B Raf/BRAF Antibody
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
大鼠, 小鼠, 人类
前体活性 :
仓鼠
应用(s) :
免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升, 人类, 大鼠, 小鼠
应用注释 :
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-B Raf/BRAF Antibody catalog # PA1848. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
BRAF
UniProt数据库编号 :
P15056
免疫原 :
A synthetic peptide corresponding to a sequence in the middle region of human B Raf (344-362aa QPFRPADEDHRNQFGQRDR), identical to the related rat and mouse sequences.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Serine/threonine-protein kinase B-raf
同义词 :
Serine/threonine-protein kinase B-raf; 2.7.11.1; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF; BRAF1, RAFB1;
蛋白名 :
Serine/threonine-protein kinase B-raf
分子量 :
84437分子量
Protein Function :
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Subcellular Localization :
Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
组织特异性 :
大脑和睾丸
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Sequence Similarities :
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
背景 :
BRAF (v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf, also known as ONCOGENE BRAF, BRAF1, RAFB1, Proto-oncogene B-Raf p94, v-Raf murine sarcoma viral oncogene homolog B1, proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1, while the protein is more formally known as serine/threonine-protein kinase B-Raf. BRAF deduced 651-amino acid protein has a calculated molecular mass of 72.5 kD. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts.B-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits