产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Cullin 2/CUL2 Antibody
目录 :
PA1877
规格 :
100μg/vial
价格 :
315美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 PA1877 图像 1
Anti-Cullin 2 antibody, PA1877, Western blotting. Recombinant Protein Detection Source: E.coli derived -recombinant human Cul2, 46.5KD (162aa tag+L150-Y394). Lane 1: Recombinant Human Cul2 Protein 10ng . Lane 2: Recombinant Human Cul2 Protein 5ng . Lane 3: Recombinant Human Cul2 Protein 2.5ng
图像 2 :
武汉博士德生物工程有限公司 PA1877 图像 2
Anti-Cullin 2 antibody, PA1877, Western blotting. WB: Rat Brain Tissue Lysate
图像 3 :
武汉博士德生物工程有限公司 PA1877 图像 3
Anti-Cullin 2 antibody, PA1877, Western blotting. Lane 1: A431 Cell Lysate. Lane 2: SMMC Cell Lysate. Lane 3: HELA Cell Lysate. Lane 4: COLO320 Cell Lysate.
产品信息
SKU号 :
PA1877
产品名称 :
Anti-Cullin 2/CUL2 Antibody
价格 :
315美元
规格 :
100μg/vial
克隆性 :
多克隆
宿主 :
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
应用(s) :
免疫印迹
应用细节 :
免疫印迹, 0.1 0.5µg/毫升, 人类, 大鼠, 小鼠
应用注释 :
WB: The detection limit for CUL2 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
描述 :
Boster Bio Anti-Cullin 2/CUL2 Antibody catalog # PA1877. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
浓度 :
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
基因名称 :
CUL2
UniProt数据库编号 :
Q13617
免疫原 :
A synthetic peptide corresponding to a sequence in the middle region of human Cullin 2 (264-280aa HECQQRMVADHLQFLHA), different from the related mouse and rat sequences by one amino acid.
形式 :
冻干
Contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
纯化 :
免疫原亲和纯化
交叉活性 :
No cross reactivity with other proteins
储存 :
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
基因全称 :
Cullin-2
同义词 :
Cullin-2; CUL-2; CUL2;
蛋白名 :
Cullin-2
分子量 :
86983分子量
Protein Function :
Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS (VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
Recommended Detection Systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
背景 :
Cullin 2 (CUL2) is a protein that in humans is encoded by the CUL2 gene. Using immunofluorescence, they showed that CUL2 is a cytosolic protein that can be translocated to the nucleus by VHL. By fluorescence in situ hybridization, Clifford et al. (1999)mapped the CUL2 gene to 10p11.2-p11.1, a region reported to show loss of heterozygosity (LOH) in several forms of human cancer, including non-clear cell renal cell carcinoma. Pause et al. (1997)suggested that CUL2 is a candidate tumor suppressor gene, as has been proposed for CUL1 (603134). Lonergan et al. (1998) demonstrated that formation of the VBC-CUL2 complexes is linked to the regulation of hypoxia-inducible mRNAs by VHL. Cul2 was one of several proteins required for degradation of a class of RNA-binding germline proteins in somatic cells of the early blastomere.
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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