产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗DDR2抗体
目录 :
PA1879
规格 :
100 ug/vial
价格 :
240美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 PA1879 图像 1
Figure 1. Western blot analysis of DDR2 using anti-DDR2 antibody (PA1879). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, . Lane 2: human placenta tissue lysates, . Lane 3: human U-87MG whole cell lysates, . Lane 4: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR2 antigen affinity purified polyclonal antibody (Catalog # PA1879) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for DDR2 at approximately 97,120-140KD. The expected band size for DDR2 is at 97KD.
产品信息
SKU号 :
PA1879
状态 :
Enabled
名称 :
抗DDR2抗体
目录名称 :
一抗, 多克隆抗体
基因名称 :
DDR2
价格 :
240美元
price various sizes :
30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
描述 :
Polyclonal antibody for TYRO10/DDR2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. TYRO10/DDR2 information: Molecular Weight: 96736 MW; Subcellular Localization: Cell membrane ; Single- pass type I membrane protein ; Tissue Specificity: Detected in osteocytes, osteoblastic cells in subchondral bone, bone lining cells, tibia and cartilage (at protein level). Detected at high levels in heart and lung, and at low levels in brain, placenta, liver, skeletal muscle, pancreas, and kidney.
short description :
Rabbit IgG polyclonal antibody for Discoidin domain-containing receptor 2(DDR2) detection. Tested with WB in Human.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
Q16832
宿主 :
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of human DDR2(834-855aa RDTKNRPSFQEIHLLLLQQGDE), different from the related mouse sequence by three amino acids.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
免疫印迹, 0.1 0.5微克/毫升, 人类
应用 :
免疫印迹
反应物种 :
人类
图像标记 :
Figure 1. Western blot analysis of DDR2 using anti-DDR2 antibody (PA1879). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HT1080 whole cell lysates, . Lane 2: human placenta tissue lysates, . Lane 3: human U-87MG whole cell lysates, . Lane 4: human U2OS whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDR2 antigen affinity purified polyclonal antibody (Catalog # PA1879) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for DDR2 at approximately 97,120-140KD. The expected band size for DDR2 is at 97KD.
背景 :
Discoidin domain-containing receptor 2, also known as CD167b (cluster of differentiation 167b), is a protein that in humans is encoded by the DDR2 gene. This gene encodes a member of the discoidin domain receptor subclass of the receptor tyrosine kinase (RTKs) protein family. RTKs play a key role in the communication of cells with their microenvironment. The encoded protein is a collagen-induced receptor that activates signal transduction pathways involved in cell adhesion, proliferation, and extracellular matrix remodeling. This protein is expressed in numerous cell types and may alos be involved in wound repair and regulate tumor growth and invasiveness. Mutations in this gene are the cause of short limb-hand type spondylometaepiphyseal dysplasia.
competitor equivalent skus :
sc 7555 sc 8989 sc 81707
研究种类 :
Cardiogenesis, Cardiovascular, Neuroscience, Neurotransmission, Receptors / Channels
同义词 :
Discoidin domain-containing receptor 2;Discoidin domain receptor 2;2.7.10.1;CD167 antigen-like family member B;Discoidin domain-containing receptor tyrosine kinase 2;Neurotrophic tyrosine kinase, receptor-related 3;Receptor protein-tyrosine kinase TKT;Tyrosine-protein kinase TYRO10;CD167b;DDR2;NTRKR3, TKT, TYRO10;
基因全称 :
Discoidin domain-containing receptor 2
分子量 :
96736分子量
protein function :
Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell differentiation, remodeling of the extracellular matrix, cell migration and cell proliferation. Required for normal bone development. Regulates osteoblast differentiation and chondrocyte maturation via a signaling pathway that involves MAP kinases and leads to the activation of the transcription factor RUNX2. Regulates remodeling of the extracellular matrix by up-regulation of the collagenases MMP1, MMP2 and MMP13, and thereby facilitates cell migration and tumor cell invasion. Promotes fibroblast migration and proliferation, and thereby contributes to cutaneous wound healing.
subcellular localization :
Cell membrane ; Single- pass type I membrane protein .
组织特异性 :
Detected in osteocytes, osteoblastic cells in subchondral bone, bone lining cells, tibia and cartilage (at protein level). Detected at high levels in heart and lung, and at low levels in brain, placenta, liver, skeletal muscle, pancreas, and kidney.
蛋白名 :
Discoidin domain-containing receptor 2
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
sequence similarities :
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
更新 :
4/15/19 6:38
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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