产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-ErbB 3/ERBB3 Antibody
目录 :
PA1880
规格 :
100 ug/vial
价格 :
240美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 PA1880 图像 1
Figure 1. Western blot analysis of ERBB3 using anti-ERBB3 antibody (PA1880). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. Lane 2: human K562 whole cell lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB3 antigen affinity purified polyclonal antibody (Catalog # PA1880) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERBB3 at approximately 180KD. The expected band size for ERBB3 is at 148KD.
产品信息
SKU号 :
PA1880
状态 :
Enabled
名称 :
Anti-ErbB 3/ERBB3 Antibody
目录名称 :
一抗, 多克隆抗体
基因名称 :
ERBB3
价格 :
240美元
price various sizes :
30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
描述 :
Polyclonal antibody for Her3/ERBB3 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. Her3/ERBB3 information: Molecular Weight: 148098 MW; Subcellular Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein; Tissue Specificity: Epithelial tissues and brain.
short description :
Rabbit IgG polyclonal antibody for Receptor tyrosine-protein kinase erbB-3(ERBB3) detection. Tested with WB in Human.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P21860
宿主 :
免疫原 :
A synthetic peptide corresponding to a sequence at the N-terminus of human ErbB 3(162-181aa DWRDIVRDRDAEIVVKDNGR).
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
免疫印迹, 0.1 0.5微克/毫升, 人类
应用 :
免疫印迹
反应物种 :
人类
前体活性 :
图像标记 :
Figure 1. Western blot analysis of ERBB3 using anti-ERBB3 antibody (PA1880). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates. Lane 2: human K562 whole cell lysates . After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB3 antigen affinity purified polyclonal antibody (Catalog # PA1880) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERBB3 at approximately 180KD. The expected band size for ERBB3 is at 148KD.
背景 :
ERBB3(V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3) also known as ONCOGENE ERBB3 or HER3, is an enzyme that in humans is encoded by the ERBB3 gene. This gene encodes a member of the epidermal growth factor receptor(EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. By in situ hybridization, Kraus et al.(1989) mapped the ERBB3 gene to chromosome 12q13. Carraway et al.(1994) demonstrated that ERBB3 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Most notably, EGFR and ErbB2 became markedly more promiscuous as the threshold was lowered, whereas ErbB3did not. Engelman et al.(2007) found that amplification of MET caused gefitinib resistance by driving ERBB3-dependent activation of phosphoinositide 3-kinase, a pathway though to be specific to EGFR/ERBB family receptors.
competitor equivalent skus :
sc 135654 sc 285 sc 285 G sc 292557 sc 415 sc 53279 sc 71067 sc 7390 sc 81454 sc 81455
研究种类 :
Apoptosis, Cell Biology, Developmental Biology, Growth Factors/Hormones, Intracellular, Kinases, Nervous System Development, Organogenesis, Protein Phosphorylation, Receptor Tyrosine Kinases, Signal Transduction, Tyrosine Kinases
同义词 :
Receptor tyrosine-protein kinase erbB-3;2.7.10.1;Proto-oncogene-like protein c-ErbB-3;Tyrosine kinase-type cell surface receptor HER3;ERBB3;HER3;
基因全称 :
Receptor tyrosine-protein kinase erbB-3
分子量 :
148098分子量
protein function :
Binds and is activated by neuregulins and NTAK. May also be activated by CSPG5.
subcellular localization :
Isoform 1: Cell membrane; Single-pass type I membrane protein.
组织特异性 :
Epithelial tissues and brain.
蛋白名 :
Receptor tyrosine-protein kinase erbB-3
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
sequence similarities :
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
更新 :
5/5/19 1:38
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
Premium Provider of Antibodies and ELISA Kits