产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Glutaredoxin 2/GLRX2 Antibody
目录 :
PA1885
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化-石蜡切片
图像
图像 1 :
武汉博士德生物工程有限公司 PA1885 图像 1
Anti-Glutaredoxin 2 antibody, PA1885, Western blotting. Lane 1: Rat Testis Tissue Lysate. Lane 2: HELA Cell Lysate. Lane 3: U87 Cell Lysate. Lane 4: NEU Cell Lysate. Lane 5: JURKAT Cell Lysate. Lane 6: MCF-7 Cell Lysate.
图像 2 :
武汉博士德生物工程有限公司 PA1885 图像 2
Anti-Glutaredoxin 2 antibody, PA1885, IHC(P). IHC(P): Rat Brain Tissue
图像 3 :
武汉博士德生物工程有限公司 PA1885 图像 3
Figure 3. IHC analysis of Glutaredoxin 2 using anti-Glutaredoxin 2 antibody (PA1885). Glutaredoxin 2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Glutaredoxin 2 Antibody (PA1885) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PA1885
状态 :
Enabled
名称 :
Anti-Glutaredoxin 2/GLRX2 Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
GLRX2
价格 :
280美元
price various sizes :
30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
描述 :
Polyclonal antibody for GRX2/GLRX2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. GRX2/GLRX2 information: Molecular Weight: 18052 MW; Subcellular Localization: Isoform 1: Mitochondrion; Tissue Specificity: Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes.
short description :
Rabbit IgG polyclonal antibody for Glutaredoxin-2, mitochondrial(GLRX2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
Q9NS18
宿主 :
免疫原 :
A synthetic peptide corresponding to a sequence in the middle region of human Glutaredoxin 2(103-119aa EYGNQFQDALYKMTGER), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5ug/ml, Human, Rat, Mouse.
应用 :
免疫组化-P, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
图像标记 :
Anti-Glutaredoxin 2 antibody, PA1885, Western blotting. Lane 1: Rat Testis Tissue Lysate. Lane 2: HELA Cell Lysate. Lane 3: U87 Cell Lysate. Lane 4: NEU Cell Lysate. Lane 5: JURKAT Cell Lysate. Lane 6: MCF-7 Cell Lysate. Anti-Glutaredoxin 2 antibody, PA1885, IHC(P). IHC(P): Rat Brain Tissue Figure 3. IHC analysis of Glutaredoxin 2 using anti-Glutaredoxin 2 antibody (PA1885). Glutaredoxin 2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Glutaredoxin 2 Antibody (PA1885) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 4. IHC analysis of Glutaredoxin 2 using anti-Glutaredoxin 2 antibody (PA1885). Glutaredoxin 2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Glutaredoxin 2 Antibody (PA1885) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
背景 :
GLRX2(Glutaredoxin-2) also known as Glutaredoxin-2, mitochondrial or GRX2, is a protein that in humans is encoded by the GLRX2 gene. Glutaredoxins(e.g., GLRX) are a family of glutathione-dependent hydrogen donors that participate in a variety of cellular redox reactions. By sequence analysis, Lundberg et al.(2001) and Gladyshev et al.(2001) identified the GLRX2 gene within a clone mapping to chromosome 1q31.2-q31.3. Lundberg et al.(2001) determined that the GLRX2 gene contains 5 exons spanning about 9.6 kb. The GLRX2B transcript uses a first exon(exon 1B) located upstream from the first exon used by the GLRX2A transcript(exon 1A), suggesting that alternative splicing generates the isoforms. Lundberg et al.(2001)assayed reductase activity in recombinant proteins of both GLRX2 isoforms. They found that both have GSH-dependent dehydroascorbate reductase activity and 2-hydroxyethyl disulfide reductase activity.
研究种类 :
Cancer, Cell Biology, Metabolism, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Oxidative Stress, Pathways And Processes, Redox Metabolism, Signal Transduction
同义词 :
Glutaredoxin-2, mitochondrial;GLRX2;GRX2;CGI-133;
基因全称 :
Glutaredoxin-2, mitochondrial
分子量 :
18052分子量
protein function :
Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
subcellular localization :
Isoform 1: Mitochondrion.
组织特异性 :
Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes.
蛋白名 :
Glutaredoxin-2, mitochondrial
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
sequence similarities :
Belongs to the glutaredoxin family.
更新 :
4/15/19 6:36
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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