抗体

Anti-Bone Sialoprotein/IBSP Antibody

PA1887

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

小鼠, 大鼠

免疫印迹, 免疫组化-石蜡切片

Anti-Bone Sialoprotein/IBSP Antibody

315美元

100μg/vial

多克隆

兔

小鼠, 大鼠

免疫组化-P, 免疫印迹

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Mouse, Rat, By Heat. Western blot, 0.1-0.5µg/ml, Rat, Mouse.

WB: The detection limit for IBSP is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Bone Sialoprotein/IBSP Antibody catalog # PA1887. Tested in IHC-P, WB applications. This antibody reacts with Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

IBSP

Q61711

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bone Sialoprotein (305-324aa SYYKGHGYEGYEGQNYYYHQ), different from the related rat sequence by one amino acid.

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Bone sialoprotein 2

Bone sialoprotein 2; Bone sialoprotein II; BSP II; Cell-binding sialoprotein; Integrin-binding sialoprotein; Ibsp;

Bone sialoprotein 2

35734分子量

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment (By similarity).

Secreted.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

IBSP (integrin-binding sialoprotein) is also known as BSP. The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

Structures