产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Bone Sialoprotein/IBSP Antibody
目录 :
PA1887
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
小鼠, 大鼠
应用 :
免疫印迹, 免疫组化-石蜡切片
图像
图像 1 :
武汉博士德生物工程有限公司 PA1887 图像 1
Anti-Bone Sialoprotein antibody, PA1887, IHC(P). IHC(P): Rat Liver Tissue
图像 2 :
武汉博士德生物工程有限公司 PA1887 图像 2
Anti-Bone Sialoprotein antibody, PA1887, Western blotting. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Kidney Tissue Lysate.
图像 3 :
武汉博士德生物工程有限公司 PA1887 图像 3
Figure 3. IHC analysis of Bone Sialoprotein using anti-Bone Sialoprotein antibody (PA1887). Bone Sialoprotein was detected in paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bone Sialoprotein Antibody (PA1887) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
产品信息
SKU号 :
PA1887
状态 :
Enabled
名称 :
Anti-Bone Sialoprotein/IBSP Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
IBSP
价格 :
280美元
price various sizes :
30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
描述 :
Polyclonal antibody for BONE SIALOPROTEIN/IBSP detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Mouse. BONE SIALOPROTEIN/IBSP information: Molecular Weight: 35734 MW; Subcellular Localization: Secreted.
short description :
Rabbit IgG polyclonal antibody for Bone sialoprotein 2(IBSP) detection. Tested with WB, IHC-P in Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
Q61711
宿主 :
免疫原 :
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bone Sialoprotein(305-324aa SYYKGHGYEGYEGQNYYYHQ), different from the related rat sequence by one amino acid.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Mouse, Rat, By Heat. Western blot, 0.1-0.5ug/ml, Rat, Mouse.
应用 :
免疫组化-P, 免疫印迹
反应物种 :
小鼠, 大鼠
图像标记 :
Anti-Bone Sialoprotein antibody, PA1887, IHC(P). IHC(P): Rat Liver Tissue Anti-Bone Sialoprotein antibody, PA1887, Western blotting. Lane 1: Rat Liver Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Kidney Tissue Lysate. Figure 3. IHC analysis of Bone Sialoprotein using anti-Bone Sialoprotein antibody (PA1887). Bone Sialoprotein was detected in paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Bone Sialoprotein Antibody (PA1887) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
背景 :
IBSP(integrin-binding sialoprotein) is also known as BSP. The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.
competitor equivalent skus :
sc 292393 sc 73497 sc 73630 sc 73634
研究种类 :
Structures
同义词 :
Bone sialoprotein 2;Bone sialoprotein II;BSP II;Cell-binding sialoprotein;Integrin-binding sialoprotein;Ibsp;
基因全称 :
Bone sialoprotein 2
分子量 :
35734分子量
protein function :
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment (By similarity).
subcellular localization :
Secreted.
蛋白名 :
Bone sialoprotein 2
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
更新 :
4/15/19 6:30
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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