抗体

Anti-Krueppel-like factor 8 KLF8 Antibody

PA1890

100μg/vial

315美元

多克隆

domestic rabbit

未共轭

Anti-Krueppel-like factor 8 KLF8 Antibody

315美元

100μg/vial

多克隆

兔

人类

免疫印迹

免疫印迹, 0.1 0.5µg/毫升, 人类

WB: The detection limit for KLF8 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Krueppel-like factor 8 KLF8 Antibody catalog # PA1890. Tested in WB applications. This antibody reacts with Human.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

KLF8

O95600

A synthetic peptide corresponding to a sequence at the N-terminus of human KLF8 (1-18aa MVDMDKLINNLEVQLNSE).

冻干

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

免疫原亲和纯化

No cross reactivity with other proteins

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Krueppel-like factor 8

Krueppel-like factor 8; Basic krueppel-like factor 3; Zinc finger protein 741; KLF8; BKLF3, ZNF741;

Krueppel-like factor 8

39314分子量

Transcriptional repressor and activator. Binds to CACCC- boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G (1) phase as a downstream target of focal adhesion kinase (FAK).

Nucleus.

Ubiquitous.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Belongs to the Sp1 C2H2-type zinc-finger protein family.

KLF8 (Krueppel-like factor 8) is a protein that in humans is encoded by the KLF8 gene. It is also named as ZNF741, or BKLF3. Kruppel-like factor proteins, including KLF8, all contain a characteristic C-terminal DNA-binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. The International Radiation Hybrid Mapping Consortium mapped the KLF8 gene to the X chromosome (WI-18614). Suske et al. (2005) stated that the human KLF8 gene maps to chromosome Xp11.21, and the mouse Klf8 gene to chromosome XF3. van Vliet et al. (2000) identified a cDNA encoding KLF8, also called ZNF741. Expression of KLF8 or only its zinc fingers showed that the fingers could bind to a CACCC box. Sequence analysis predicted that the 359-amino acid protein contains a nuclear localization signal upstream of the zinc fingers, a valine-rich hydrophobic stretch, and a pro-val-asp-leu-ser (PVDLS) motif. Yeast 2-hybrid and GST pull-down analysis demonstrated that CTBP2 interacts with KLF8 through the PVDLS motif.

Domain Families, Epigenetics and Nuclear Signaling, Krueppel Like Factor, Transcription, Zinc Finger