产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
Anti-Nucleophosmin/NPM1 Antibody
目录 :
PA1931
规格 :
100 ug/vial
价格 :
280美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
图像
图像 1 :
武汉博士德生物工程有限公司 PA1931 图像 1
Anti-Nucleophosmin antibody, PA1931, Western blotting. Lane 1: Rat Testis Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: HELA Cell Lysate. Lane 4: U87 Cell Lysate. Lane 5: A549 Cell Lysate. Lane6: SMMC Cell Lysate.
图像 2 :
武汉博士德生物工程有限公司 PA1931 图像 2
Anti-Nucleophosmin antibody, PA1931, IHC(P). IHC(P): Human Intestinal Cancer Tissue
图像 3 :
武汉博士德生物工程有限公司 PA1931 图像 3
Anti-Nucleophosmin antibody, PA1931, ICC. ICC: HELA Cell
产品信息
SKU号 :
PA1931
状态 :
Enabled
名称 :
Anti-Nucleophosmin/NPM1 Antibody
目录名称 :
一抗, 多克隆抗体, 免疫组化免疫细胞化学IF抗体
基因名称 :
NPM1
价格 :
280美元
price various sizes :
30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
描述 :
Polyclonal antibody for B23/NPM1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. B23/NPM1 information: Molecular Weight: 32575 MW; Subcellular Localization: Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
short description :
Rabbit IgG polyclonal antibody for Nucleophosmin(NPM1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
P06748
宿主 :
免疫原 :
A synthetic peptide corresponding to a sequence at the N-terminus of human Nucleophosmin(22-41aa ELKADKDYHFKVDNDENEHQ), identical to the related rat and mouse sequences.
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
Immunocytochemistry , 0.5-1ug/ml, Human, Mouse, Rat. Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Rat, Mouse, By Heat. Western blot, 0.1-0.5ug/ml, Human, Rat, Mouse.
应用 :
免疫组化, 免疫细胞化学, 免疫印迹
反应物种 :
人类, 小鼠, 大鼠
前体活性 :
仓鼠
图像标记 :
Anti-Nucleophosmin antibody, PA1931, Western blotting. Lane 1: Rat Testis Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: HELA Cell Lysate. Lane 4: U87 Cell Lysate. Lane 5: A549 Cell Lysate. Lane6: SMMC Cell Lysate. Anti-Nucleophosmin antibody, PA1931, IHC(P). IHC(P): Human Intestinal Cancer Tissue Anti-Nucleophosmin antibody, PA1931, ICC. ICC: HELA Cell Anti-Nucleophosmin antibody, PA1931, IHC(P). IHC(P): Rat Spleen Tissue
背景 :
NPM1(Nucleophosmin/Nucleoplasmin family, member1), also known as NPM, nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene. The NPM1 gene maps to chromosome 5q35. Chan et al.(1989) found that nucleophosmin is a nucleolar phosphoprotein that is more abundant in tumor cells than in normal resting cells. Stimulation of the growth of normal cells, e.g., mitogen activation of B lymphocytes, was accompanied by an increase in nucleophosmin protein level. They stated that nucleophosmin is likely involved in the assembly of ribosomal proteins into ribosomes. Electron microscopic study indicated that nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.
competitor equivalent skus :
sc 271737 sc 32256 sc 47725 sc 53175 sc 5564 sc 56622 sc 6013 R sc 65943 sc 70392
研究种类 :
Cancer, Cell Type Marker, Cell Type Markers, Intracellular Signaling, Kinases, Neuron Marker, Neuroscience, Neurotransmission, Oncoproteins, Oncoproteins/Suppressors, Receptors / Channels, Tags & Cell Markers, Tumor Associated
同义词 :
Nucleophosmin;NPM;Nucleolar phosphoprotein B23;Nucleolar protein NO38;Numatrin;NPM1;NPM;
基因全称 :
Nucleophosmin
分子量 :
32575分子量
protein function :
Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double- stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation.
subcellular localization :
Nucleus, nucleolus. Nucleus, nucleoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.
蛋白名 :
Nucleophosmin
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
sequence similarities :
Belongs to the nucleoplasmin family.
更新 :
1/28/19 19:39
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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