产品简要
公司名称 :
武汉博士德生物工程有限公司
产品类型 :
抗体
产品名称 :
抗NFkB p100/p52/NFKB2抗体
目录 :
PA1946-1
规格 :
100 ug/vial
价格 :
240美元
克隆性 :
多克隆
宿主 :
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹
图像
图像 1 :
武汉博士德生物工程有限公司 PA1946-1 图像 1
Anti-NFkB p100/p52 antibody, PA1946-1, Western blotting. Lane 1: HELA Cell Lysate. Lane 2: JURKAT Cell Lysate.
产品信息
SKU号 :
PA1946-1
状态 :
Enabled
名称 :
抗NFkB p100/p52/NFKB2抗体
目录名称 :
一抗, 多克隆抗体
基因名称 :
NFKB2
价格 :
240美元
price various sizes :
30ug sample size / $99 100ug / $240 100ug+Free HRP Secondary BA1054 / $240 100ug+Free Biotin Secondary BA1003 / $240
克隆性 :
多克隆
浓度 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
共轭标签 :
No
contents :
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
描述 :
Polyclonal antibody for NFKB P100/NFKB2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: WB. Reactive species: Human. NFKB P100/NFKB2 information: Molecular Weight: 96749 MW; Subcellular Localization: Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
short description :
Rabbit IgG polyclonal antibody for Nuclear factor NF-kappa-B p100 subunit(NFKB2) detection. Tested with WB in Human.
规格 :
100 ug/vial
sample size available :
30ug for $99, contact us for details
UniProt数据库编号 :
Q00653
宿主 :
免疫原 :
A synthetic peptide corresponding to a sequence at the N-terminus of human NFkB p100(1-21aa MESCYNPGLDGIIEYDDFKLN).
形式 :
冻干
纯化 :
免疫原亲和纯化
储存 :
At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.
交叉活性 :
No cross reactivity with other proteins
重组 :
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
应用细节 :
免疫印迹, 0.1 0.5微克/毫升, 人类
应用 :
免疫印迹
反应物种 :
人类
图像标记 :
Anti-NFkB p100/p52 antibody, PA1946-1, Western blotting. Lane 1: HELA Cell Lysate. Lane 2: JURKAT Cell Lysate.
背景 :
NFKB2(Nuclear Factor Kappa-B, Subunit 2), also known as NFKB or p52/p100 SUBUNIT, is a protein that in humans is encoded by the NFKB2 gene. Liptay et al.(1992) mapped the gene for what they called the p49/p100 subunit of NFKB(NFKB2) to chromosome 10 by Southern blot analysis of panels of human/Chinese hamster cell hybrids. By fluorescence in situ hybridization(FISH), they confirmed the localization and mapped the gene with greater resolution to 10q24. NFKB2 appears to be the same as LYT10. Claudio et al.(2002) showed that bone marrow(BM) cells from Nfkb2-deficient mice, but not Nfkb1-deficient mice, failed to increase relative and total IgD-positive transitional-1(T1) stage B cells in response to Baff. In vivo, however, Nfkb2-deficient mice did generate mature B cells, but at reduced numbers. Mice of the aly/aly strain, which are naturally deficient in Nik, and mice of the A/WySNJ strain, which have a mutation in Baffr, also failed to produce T1 B cells in response to Baff. Baff stimulation enhanced expression of Bcl2 in T1 B cells, thereby promoting B-cell survival, and caused the processing of the p100 form of Nfkb2 to p52, which again required Baffr and Nik, but not Nemo(IKKG). Immunoblot analysis showed that BM cells contained primarily p100.
competitor equivalent skus :
sc 101742 sc 101743 sc 298 sc 298 G sc 298 X sc 7386 sc 7386 X sc 848 sc 848 X
研究种类 :
Apoptosis, Apoptotic Markers, Cancer, Cell Biology, Cell Death, Epigenetics And Nuclear Signaling, Intracellular, Nfkb Pathway, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway, Transcription
同义词 :
Nuclear factor NF-kappa-B p100 subunit;DNA-binding factor KBF2;H2TF1;Lymphocyte translocation chromosome 10 protein;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2;Oncogene Lyt-10;Lyt10;Nuclear factor NF-kappa-B p52 subunit;NFKB2;LYT10;
基因全称 :
Nuclear factor NF-kappa-B p100 subunit
分子量 :
96749分子量
protein function :
NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF- kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14- activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. In concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK- ARNTL/BMAL1 heterodimer.
subcellular localization :
Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
蛋白名 :
Nuclear factor NF-kappa-B p100 subunit
recommended detection systems :
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
sequence similarities :
Contains 7 ANK repeats.
更新 :
1/28/19 19:39
公司信息
武汉博士德生物工程有限公司
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
http://www.boster.com.cn
925.485.4527
公司总部: 美国
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